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Genomic characterization of pararetroviral sequences in wild Dahlia spp. in natural habitats

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Abstract

The genome structure and organization of endogenous caulimovirus sequences from dahlia (Dahlia spp), dahlia mosaic virus (DMV)-D10 from three wild species, D. coccinea (D10-DC), D. sherffii (D10-DS) and D. tenuicaulis (D10-DT), were determined and compared to those from cultivated species of dahlia, D. variabilis (DvEPRS). The complete ca. 7-kb dsDNA genomes of D10-DC, D10-DS, and D10-DT had a structure and organization typical of a caulimovirus and shared 89.3 to 96.6% amino acid sequence identity in various open reading frames (ORF) when compared to DvEPRS. The absence of the aphid transmission factor and the truncated coat protein fused with the reverse transcriptase ORF were common among these DMV-D10 isolates from wild Dahlia species.

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Acknowledgments

This research was supported in part by the Samuel and Patricia Smith Endowment for Dahlia Virus Research, created by the American Dahlia Society, and funding from the USDA Northwest Nursery Crop Research Center. S. Eid was supported, in part, by a graduate student research assistantship from the WSU-Agricultural Research Center. PPNS No. 0574, Department of Plant Pathology, College of Agricultural, Human and Natural Resource Sciences, Agricultural Research Center, Project # WNPO 0545, Washington State University, Pullman, WA 99164-6430, USA. Field work in Mexico was supported, in part, by KY NSF EPSCoR grant no. 3046884400-06-403 to DES. DES expresses her appreciation for field assistance from Jeffrey R. Bacon, Universidad Juárez del Estado de Durango. Support from the Plant Virus Ecology Network NSF RCN AWARD IOS-0639139 is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, PO Box 646430, Pullman, WA, 99164-6430, USA

    S. Eid, C. V. Almeyda, K. L. Druffel & H. R. Pappu

  2. Department of Biological Sciences, Murray State University, Murray, KY, USA

    D. E. Saar

Authors
  1. S. Eid
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  2. C. V. Almeyda
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  3. D. E. Saar
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  4. K. L. Druffel
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  5. H. R. Pappu
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Corresponding author

Correspondence to H. R. Pappu.

Additional information

The sequences described here were deposited in GenBank with the following accession numbers: HQ261756, HQ261757, HQ261758, HQ261759, HQ336476, HQ336477, HQ336478, HQ336479, HQ416675, HQ416676, HQ416677, and HQ416678.

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Eid, S., Almeyda, C.V., Saar, D.E. et al. Genomic characterization of pararetroviral sequences in wild Dahlia spp. in natural habitats. Arch Virol 156, 2079–2084 (2011). https://doi.org/10.1007/s00705-011-1076-y

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