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An infectious full-length cDNA clone of potato virus YNTN-NW, a recently reported strain of PVY that causes potato tuber necrotic ringspot disease

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Abstract

A full-length infectious cDNA clone of potato virus Y (PVY) was constructed based on an isolate of the PVYNTN-NW strain, SYR-II-2-8, which is able to cause potato tuber necrotic ringspot disease (PTNRD). Silent point mutations were introduced to modify sequences similar to the prokaryotic promoter elements detected at the 5’ terminus of the P3 coding region of SYR-II-2-8. This, along with modification of the growing conditions of E. coli, led to the successful construction of a stable full-length infectious cDNA clone, named p2-8C3. The biological properties of p2-8C3 were identical to those of SYR-II-2-8, both of which induced PTNRD in potato, veinal necrosis in tobacco, and similar symptoms in the potato cultivars inoculated.

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Acknowledgments

The first author gratefully acknowledges the Japan Society for the Promotion of Science (JSPS) for granting research support to study potato viruses in Syria. Authors wish to thank Dr. Atsushi Ohkawa for useful discussions and suggestions.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya, 321-8505, Japan

    M. Chikh Ali & T. Natsuaki

  2. General Organization for Seed Multiplication (GOSM), Aleppo, Syria

    M. Chikh Ali

  3. Department of Crop Science, Faculty of Agriculture, Aleppo University, Aleppo, Syria

    A. Said Omar

Authors
  1. M. Chikh Ali
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  2. A. Said Omar
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  3. T. Natsuaki
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Correspondence to M. Chikh Ali.

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Chikh Ali, M., Said Omar, A. & Natsuaki, T. An infectious full-length cDNA clone of potato virus YNTN-NW, a recently reported strain of PVY that causes potato tuber necrotic ringspot disease. Arch Virol 156, 2039–2043 (2011). https://doi.org/10.1007/s00705-011-1062-4

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  • DOI: https://doi.org/10.1007/s00705-011-1062-4

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