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Human cytomegalovirus latency-associated protein LUNA is expressed during HCMV infections in vivo

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Abstract

Human cytomegalovirus (HCMV) latency is poorly understood. We previously described a novel HCMV latency-associated transcript, UL81-82ast, coding for a protein designated LUNA (latency unique natural antigen). The aim of this study was to confirm the presence of LUNA in HCMV-seropositive donors. Standard co-immunoprecipitation and ELISA assays were used to detect antibodies against the LUNA protein in the sera of HCMV-seropositive donors. Specific antibodies against LUNA were detected in all HCMV-seropositive donors but in none of the seronegative donors. These data confirm that LUNA is expressed during in vivo infections and is capable of eliciting an immune response.

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Acknowledgments

We would like to thank Karen Schlauch for aiding in the statistics portion of this project. This project was supported by NIH grant number HL63470.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institut de recherches cliniques de Montréal (IRCM), 110, avenue des Pins Ouest, Montreal, QC, H2W 1R7, Canada

    Mariana G. Bego

  2. Department of Microbiology and Graduate Program in Cell and Molecular Biology, University of Nevada, 1664 N Virginia, MS320, Reno, NV, 89557, USA

    Lisa R. Keyes & Stephen C. St Jeor

  3. The Cleveland Clinic, Taussig Cancer Center, 9500 Euclid Avenue, Cleveland, OH, 44195, USA

    Jarek Maciejewski

Authors
  1. Mariana G. Bego
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  2. Lisa R. Keyes
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  3. Jarek Maciejewski
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  4. Stephen C. St Jeor
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Corresponding author

Correspondence to Stephen C. St Jeor.

Additional information

M. G. Bego and L. R. Keyes contributed equally.

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Bego, M.G., Keyes, L.R., Maciejewski, J. et al. Human cytomegalovirus latency-associated protein LUNA is expressed during HCMV infections in vivo. Arch Virol 156, 1847–1851 (2011). https://doi.org/10.1007/s00705-011-1027-7

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  • DOI: https://doi.org/10.1007/s00705-011-1027-7

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