Skip to main content

Advertisement

SpringerLink
Log in

A novel gammaherpesvirus isolated from a black-tailed prairie dog (Cynomys ludovicianus)

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A new gammaherpesvirus, tentatively named cynomys herpesvirus 1 (CynGHV-1), was isolated from a black-tailed prairie dog (Cynomys ludovicianus). CynGHV-1 replicated cytopathogenically to moderate titers in various cell lines. Ten kb of the CynGHV-1 genome was sequenced using degenerate PCR and genomic cloning. Sequence similarities were found to different genes from known gammaherpesviruses. Phylogenetic analysis suggested that CynGHV-1 was in fact a novel virus closely related to representatives of different genera and unclassified members of the subfamily Gammaherpesvirinae. However, CynGHV-1 could not be assigned to any particular genus and therefore remains unclassified.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Ackerman M (2004) Herpesviruses, a brief overview. Methods Mol Biol 256:199–219

    Google Scholar 

  2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  3. Ehlers B, Dural G, Yasmum N, Lembo T, de Thoisy B, Ryser-Degiorgis MP, Ulrich RG, McGeoch DJ (2008) Novel mammalian herpesviruses and lineages within the gammaherpesvirinae: cospeciation and interspecies transfer. J Virol 82:3509–3516

    Article  PubMed  CAS  Google Scholar 

  4. Guindon S, Gascuel O (2003) A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704

    Article  PubMed  Google Scholar 

  5. Goloboff PA, Farris JS (2001) Methods for quick consensus estimations. Cladistics 17:S26–S34

    Article  Google Scholar 

  6. Hayward GS, Zong JC (2007) Modern evolutionary history of the human KSHV genome. Curr Top Microbiol Immunol 3:1–42

    Article  Google Scholar 

  7. Huelsenbeck JP, Ronquist F (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754–755

    Article  PubMed  CAS  Google Scholar 

  8. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066

    Article  PubMed  CAS  Google Scholar 

  9. Katoh K, Asimenos G, Toh H (2009) Multiple alignment of DNA sequences with MAFFT. Methods Mol Biol 537:39–64

    Article  PubMed  CAS  Google Scholar 

  10. Keane TM, Creevey CJ, Pentony MM, Naughton TJ, Mclnerney JO (2006) Assessment of methods for amino acid matrix selection and their use on empirical data shows that ad hoc assumptions for choice of matrix are not justified. BMC Evol Biol 6:29

    Article  PubMed  Google Scholar 

  11. Kruger JM, Venta PJ, Swenson CL, Syring R, Gibbons-Burgener SN, Richter M, Maes RK (2000) Prevalence of bovine herpesvirus-4 infection in cats in Central Michigan. J Vet Intern Med 14:593–597

    Article  PubMed  CAS  Google Scholar 

  12. Martinez-Guzman D, Rickabaugh T, Wu TT, Brown H, Cole S, Song MJ, Tong L, Sun R (2003) Transcription program of murine gammaherpesvirus 68. J Virol 77:10488–10503

    Article  PubMed  CAS  Google Scholar 

  13. Martins AN, Medeiros SO, Simonetti JP, Schatzmayr HG, Tanuri A, Brindeiro RM (2008) Phylogenetic and genetic analysis of feline immunodeficiency virus gag, pol, and env genes from domestic cats undergoing nucleoside reverse transcriptase inhibitor treatment or treatment-naive cats in Rio de Janeiro, Brazil. J Virol 82:7863–7874

    Article  PubMed  CAS  Google Scholar 

  14. McGeoch DJ, Gatherer D, Dolan A (2005) On phylogenetic relationships among major lineages of the gammaherpesvirinae. J Gen Virol 86:307–316

    Article  PubMed  CAS  Google Scholar 

  15. McGill R, Tukey JW, Larsen WA (1978) Variations of box plots. Am Stat 32:12–16

    Article  Google Scholar 

  16. Melendez LV, Daniel MD, Barahona HH, Fraser CE, Hunt RD, Garcia FG (1971) New herpesviruses from South American monkeys. Preliminary report. Lab Anim Sci 21:1050–1054

    PubMed  CAS  Google Scholar 

  17. Philippe H, Snell E, Bapteste E, Lopez P, Holland P, Casane D (2004) Phylogenomics of eukaryotes: impact of missing data on large alignments. Mol Biol Evol 21:1740–1752

    Article  PubMed  CAS  Google Scholar 

  18. Ramer JC, Garber RL, Steele KE, Boyson JF, O’Rourke C, Thomson JA (2000) Fatal lymphoproliferative disease associated with a novel gammaherpesvirus in a captive population of common marmosets. Lab Anim Sci 50:59–68

    CAS  Google Scholar 

  19. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  PubMed  CAS  Google Scholar 

  20. Rose TM, Strand KB, Schultz ER, Schaefer G, Rankin GW Jr, Thouless ME, Tsai CC, Bosch ML (1997) Identification of two homologs of the Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8) in retroperitoneal fibromatosis of different macaque species. J Virol 71:4138–4144

    PubMed  CAS  Google Scholar 

  21. Shin YC, Jones LR, Manrique J, Lauer W, Carville A, Mansfield KG, Desrosiers RC (2010) Glycoprotein gene sequence variation in rhesus monkey rhadinovirus. Virology 400:175–186

    Article  PubMed  CAS  Google Scholar 

  22. Taylor SK, Vieira VG, Williams ES, Pilkington R, Fedorchak SL, Mills KW, Cavender JL, Boerger-Fields AM, Moore RE (1996) Infectious keratoconjunctivitis in free-ranging mule deer (Odocoileus hemionus) from Zion national Park, Utah. J Wildl Dis 32:326–330

    PubMed  CAS  Google Scholar 

  23. VanDevanter DR, Warrener P, Bennett L, Schultz ER, Coulter S, Garber RL, Rose TM (1996) Detection and analysis of diverse herpesviral species by consensus primer PCR. J Clin Microbiol 34:1666–1671

    PubMed  CAS  Google Scholar 

  24. Wiens JJ (2006) Missing data and the design of phylogenetic analyses. J Biomed Inform 39:34–42

    Article  PubMed  CAS  Google Scholar 

  25. Wu GA, Jun S, Sims GE, Kim SH (2009) Whole-proteome phylogeny of large dsDNA virus families by an alignment-free method. Proc Natl Acad Sci USA 106:12826–12831

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank Mrs. Carol E. Hearne for the electron microscopy data. We are also very grateful to Dr. Geoff J. Letchworth for his advice and enthusiasm throughout the duration of the project as well as critical review of this manuscript. The authors also wish to thank Dr. Charles H. Calisher and Dr. J. Jeffrey Root, (CSU, Fort Collins) for providing the prairie dog serum samples. Bioinformatic analysis by CT-R was supported by NIH Grant P20 RR016474 from the INBRE Program of the National Center for Research Resources.

Author information

Author notes

  1. Christian Tellgren-Roth

    Present address: Rudbecklaboratoriet, Department of Genetics and Pathology, Uppsala University, 751 85, Uppsala, Sweden

  2. Ana C. Bratanich

    Present address: Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Avenida Chorroarin 280, 1427, Buenos Aires, Argentina

Authors and Affiliations

  1. Department of Veterinary Science, University of Wyoming, Laramie, WY, 82070, USA

    Brandy Nagamine, Jacqueline Cavender & Ana C. Bratanich

  2. Center for Rural Health Research and Education, University of Wyoming, Laramie, WY, 82070, USA

    Christian Tellgren-Roth

  3. Division of Molecular Biology, Estación de Fotobiología Playa Unión, CC 15, 9103, Playa Unión, Chubut, Argentina

    Leandro Jones

Authors
  1. Brandy Nagamine
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leandro Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christian Tellgren-Roth
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jacqueline Cavender
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ana C. Bratanich
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ana C. Bratanich.

Additional information

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are EU863271-863275 and EU863200.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Figure S1.

Panoramic view alignment of complete pol aminoacid sequences of CynGHV-1 and similar sequences from GenBank obtained by Blast analysis. Alignment positions for which data was available were given a colored background. Sequence positions corresponding to gaps were given a black background (and thus are visualized as black boxes). Sequence positions that are missing (because of disparities in the length of sequences) are indicated with question marks (?) and have no background. (PDF 1333 kb)

Figure S2.

Parsimony tree of complete pol amino acid sequences of CynGHV-1 and similar sequences from GenBank as shown in Fig S1. Numbers on tree nodes indicate bootstrap (n=100) support. Branches with bootstrap supports lower than 50 were collapsed. Maximum Likelihood and Bayesian analyses (not shown) gave similar results. Branch lengths are proportional to the number of amino acid substitutions (bar = 10 substitutions). (TIFF 14080 kb)

Supplementary material 3 (DOC 208 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nagamine, B., Jones, L., Tellgren-Roth, C. et al. A novel gammaherpesvirus isolated from a black-tailed prairie dog (Cynomys ludovicianus). Arch Virol 156, 1835–1840 (2011). https://doi.org/10.1007/s00705-011-1024-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-011-1024-x

Keywords

Search

Navigation