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Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction

Archives of Virology volume 156, pages 1655–1660 (2011)Cite this article

Abstract

Dobrava (DOBV) hantaviruses belong to the genus Hantavirus, family Bunyaviridae, and are carried by yellow-necked and striped field mice. The goal of this study was to detect DOBV using serological and genetic methods in Apodemus rodents in Hungary and in northern Croatia. During the study period, a total of 125 Apodemus sp. (67 A. agrarius, 58 A. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rRT-PCR and/or ELISA. We conclude that the prevalence of DOBV is much higher than previously anticipated. The simultaneous use of molecular and serological techniques provides a highly reliable way to detect hantavirus infections.

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References

  1. Araki K, Yoshimatsu K, Ogino M, Ebihara H, Lundkvist A, Kariwa H, Takashima I, Arikawa J (2001) Truncated hantavirus nucleokapsid proteins for serotyping Hantaan, Seoul, and Dobrava Hantavirus infections. J Clin Microbiol 39:2397–2404

    PubMed  Article  CAS  Google Scholar 

  2. Clement J, McKenna P, Leirs H, Verhagen R, Lefevre A et al (1994) Hantavirus infections in rodents. In: Osterhaus A (ed) Virus infections of rodents and lagomorphs. Elsevier, Amsterdam, pp 295–316

    Google Scholar 

  3. Clement J, Heyman P, Colson P, Groeneveld PH (1996) Spread of hantavirus infections in Europe. Lancet 347:771

    PubMed  Article  CAS  Google Scholar 

  4. Easterbrook JD, Klein SL (2008) Immunological mechanisms mediating hantavirus persistence in rodent reservoirs. PLoS Pathog 4(11):e1000172

    PubMed  Article  Google Scholar 

  5. Faludi G, Ferenczi E (1995) Serologically verified hantavirus infections in Hungary. Acta Microbiol Immunol Hung 42:419–426

    PubMed  CAS  Google Scholar 

  6. Gavrilovskaya IN, Apekina NS, Myasnikov YuA, Bernshtein AD, Ryltseva EV, Gorbachkova EA, Chumakov MP (1983) Features of circulation of hemorrhagic fever with renal syndrome (HFRS) virus among small mammals in the European USSR. Arch Virol 75:313–316

    PubMed  Article  CAS  Google Scholar 

  7. Jakab F, Horváth G, Ferenczi E, Sebők J, Varecza Z, Szűcs G (2007) Detection of Dobrava hantaviruses in Apodemus agrarius mice in the Transdanubian region of Hungary. Virus Res 128:149–152

    PubMed  Article  CAS  Google Scholar 

  8. Jakab F, Sebők J, Ferenczi E, Horváth G, Szűcs G (2007) First detection of Dobrava hantavirus from a patient with severe haemorrhagic fever with renal syndrome by SYBR Green-based real time RT-PCR. Scand J Infect Dis 39:902–906

    PubMed  Article  CAS  Google Scholar 

  9. Jakab F, Horváth G, Ferenczi E, Sebők J, Szűcs G (2008) First detection of Tula hantaviruses in Microtus arvalis voles in Hungary. Arch Virol 153:2093–2096

    PubMed  Article  CAS  Google Scholar 

  10. Kang HJ, Arai S, Hope AG, Song JW, Cook JA, Yanagihara R (2009) Genetic diversity and phylogeography of Seewis virus in the Eurasian common shrew in Finland and Hungary. Virol J 6:208

    PubMed  Article  Google Scholar 

  11. Kang HJ, Bennett SN, Sumibcay L, Arai S, Hope AG, Mocz G, Song JW, Cook JA, Yanagihara R (2009) Evolutionary insights from a genetically divergent hantavirus harbored by the European common mole (Talpa europaea). PLoS One 4(7):e6149

    PubMed  Article  Google Scholar 

  12. Klempa B, Tkachenko EA, Dzagurova TK, Yunicheva YV, Morozov VG, Okulova NM, Slyusareva GP, Smirnov A, Kruger DH (2008) Hemorrhagic fever with renal syndrome caused by 2 lineages of Dobrava hantavirus, Russia. Emerg Infect Dis 14:617–625

    PubMed  Article  Google Scholar 

  13. Maes P, Klempa B, Clement J, Matthijnssens J, Gajdusek DC, Krüger DH, Van Ranst M (2009) A proposal for new criteria for the classification of hantaviruses, based on S and M segment protein sequences. Infect Genet Evol 9:813–820

    PubMed  Article  CAS  Google Scholar 

  14. Nemirov K, Vapalathi O, Lundkvist Å, Vasilenco V, Golovljova I, Plyusnina A, Niemimaa J, Laakkinen J, Henttonen H, Vaheri A, Plyusnin A (1999) Isolation and characterisation of Dobrava hantavirus carried by the striped field mouse (Apodemus flavicollis) in Estonia. J Gen Virol 80:371–379

    PubMed  CAS  Google Scholar 

  15. Plyusnina A, Ferenczi E, Rácz RG, Nemirov K, Lundkvist A, Vaheri A, Vapalathi O, Plyusnin A (2009) Co-circulation of three pathogenic hantaviruses: Puumala, Dobrava, and Saaremaa in Hungary. J Med Virol 81:2045–2052

    PubMed  Article  Google Scholar 

  16. Sjölander KB, Golovljova I, Vasilenko V, Plyusnin A, Lundkvist A (2002) Serological divergence of Dobrava and Saaremaa hantaviruses: evidence for two distinct serotypes. Epidemiol Infect 128:99–103

    PubMed  Google Scholar 

  17. Tkachenko EA, Ivanov AP, Donets MA, Miasnikov YA, Ryltseva EV, Gaponova LK, Bashkirtsev VN, Okulova NM, Drozdov SG, Slonova RA et al (1983) Potential reservoir and vectors of haemorrhagic fever with renal syndrome (HFRS) in the U.S.S.R. Ann Soc Belg Med Trop 63:267–269

    PubMed  CAS  Google Scholar 

  18. Vapalahti O, Mustonen J, Lundkvist A, Henttonen H, Plyusnin A, Vaheri A (2003) Hantavirus infections in Europe. Lancet Infect Dis 3:653–661

    PubMed  Article  Google Scholar 

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Acknowledgment

This study was supported by the Hungarian Scientific Research Fund (OTKA; PD77977) and Science, Please! Research Team on Innovation (SROP-4.2.2/08/1/2008-0011) project. The study was performed during the period of the “Bolyai János” Research Fellowship of Ferenc Jakab and Krisztián Bányai. We also would like to give special thanks to Dr. Emőke Ferenczi and Kaposi Tamásné (National Center for Epidemiology, Department of Viral Diagnostics, Reference Laboratory of Viral Zoonozes, Budapest, Hungary). We thank Tibor Pál for his comments and assistance in editing of the manuscript.

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Affiliations

  1. Virological Research Group, Faculty of Sciences, Institute of Biology, University of Pécs, Ifjúság út 6, 7624, Pecs, Hungary

    Viktória Németh, Mónika Madai, Alexandra Maráczi, Bálint Bérczi, Miklós Oldal & Ferenc Jakab

  2. Virological Laboratory, Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pecs, Hungary

    Ferenc Jakab

  3. Department of Animal Ecology, Faculty of Sciences, Institute of Biology, University of Pécs, Pecs, Hungary

    Győző Horváth

  4. Department of Medical Genetics, Medical School, University of Pécs, Pecs, Hungary

    Péter Kisfali

  5. Veterinary Medical Research Institute Hungarian Academy of Sciences, Budapest, Hungary

    Krisztián Bányai

Authors
  1. Viktória Németh
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  2. Mónika Madai
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  3. Alexandra Maráczi
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  4. Bálint Bérczi
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  5. Győző Horváth
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  6. Miklós Oldal
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  7. Péter Kisfali
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  8. Krisztián Bányai
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  9. Ferenc Jakab
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Correspondence to Ferenc Jakab.

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Németh, V., Madai, M., Maráczi, A. et al. Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction. Arch Virol 156, 1655–1660 (2011). https://doi.org/10.1007/s00705-011-1013-0

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  • DOI: https://doi.org/10.1007/s00705-011-1013-0

Keywords

  • Nucleocapsid Protein
  • Hemorrhagic Fever With Renal Syndrome
  • Nephropathia Epidemica
  • Striped Field Mouse
  • Apodemus Mouse