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Identification of a phylogenetically distinct orthobunyavirus from group C

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Abstract

Apeu virus (APEUV) (family Bunyaviridae, genus Orthobunyavirus) was plaque purified and characterised by serological and molecular analysis. Neutralising assays confirmed cross-reactivity between purified APEUV clones and the Caraparu virus complex of group C orthobunyaviruses. Partial sequencing of the L, M and S segments of one APEUV clone (APEUV-CL5) was carried out. A phylogenetic tree constructed with the L amino acid sequences clustered APEUV-CL5 within the genus Orthobunyavirus, confirming its serological classification. Analysis of M segment sequences clustered APEUV-CL5 in the Caraparu virus complex (Group C), in agreement with serological tests and previous molecular characterisation. However, the sequence of the nucleocapsid gene (N) gave low identity values when compared to those of the group C viruses. The phylogenetic tree based on N nucleotide sequences clustered APEUV-CL5 next to the California and Bwamba groups. This remarkable S nucleotide variability suggests that APEUV-CL5 could be a genetic reassortant and that this evolutionary mechanism is present in the history of the group C viruses.

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Acknowledgments

The authors are grateful to colleagues from Virus Laboratory (ICB-UFMG) for their technical and scientific supports. This work was supported by research grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG). P.C.P. Ferreira, E.G. Kroon, and C.A. Bonjardim are CNPq researchers. We thank Dr. G.S. Trindade and Dr. Jaquelline G. Oliveira for helpful scientific discussions. We thank the Laboratory of Biodiversity and Molecular Evolution (ICB-UFMG), where sequences were determined.

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Authors and Affiliations

  1. Laboratório de Bioquímica Metabólica, Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil

    Cintia Lopes de Brito Magalhães

  2. Laboratório de Virologia, Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil

    Betânia Paiva Drumond

  3. Seção Regional de Criminalística da 2ª Delegacia Regional de Polícia do 5º Departamento de Polícia Civil do Estado de Minas Gerais, Araxá, MG, Brazil

    Renata Franco Vianna Novaes

  4. Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Bárbara Rezende Quinan

  5. Laboratório de Microbiologia, Universidade Federal de São João Del-Rei, Ouro Branco, MG, Brazil

    José Carlos de Magalhães

  6. Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, CEP 31270-901, Brazil

    Cintia Lopes de Brito Magalhães, Betânia Paiva Drumond, Renata Franco Vianna Novaes, Bárbara Rezende Quinan, João Rodrigues dos Santos, Carla do Amaral Pinto, Marieta Torres de Abreu Assis, Cláudio Antônio Bonjardim, Erna Geessien Kroon & Paulo César Peregrino Ferreira

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  1. Cintia Lopes de Brito Magalhães
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Corresponding author

Correspondence to Paulo César Peregrino Ferreira.

Additional information

The S, M, and L nucleotide sequences reported here are available in GenBank under the accession numbers FJ859037, FJ859038, and FJ859039, respectively.

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de Brito Magalhães, C.L., Drumond, B.P., Novaes, R.F.V. et al. Identification of a phylogenetically distinct orthobunyavirus from group C. Arch Virol 156, 1173–1184 (2011). https://doi.org/10.1007/s00705-011-0976-1

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