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Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana

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Abstract

Deep sequencing of the polyadenylated transcriptome of a wild plant of Hardenbergia comptoniana was used to distinguish with high support complete genome sequences of two distinct isolates of Hardenbergia mosaic virus (HarMV) co-infecting it. Isolates shared 82.0% nucleotide (nt) and 86.7% amino acid (aa) identity. Isolate 57.1 (9,628 nt) had a deletion of 17 contiguous aa within the conserved core region of the coat protein (CP), while isolate 57.2 (9,675 nt) had a wild-type CP. Overall, HarMV genomes accounted for 10.69% of total transcripts sequenced, and the mutant virus genome was 23.1% more highly expressed than the wild-type genome.

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Acknowledgments

This work was supported by the Australian Research Council (LP0882671) and the Murdoch University Institutes for Crop and Plant Science Research and for Sustainable Ecosystems Research.

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  1. Plant Virus Section, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA, 6150, Australia

    Stephen J. Wylie & Michael G. K. Jones

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Correspondence to Stephen J. Wylie.

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Wylie, S.J., Jones, M.G.K. Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana . Arch Virol 156, 1251–1255 (2011). https://doi.org/10.1007/s00705-011-0965-4

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