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Ranunculus latent virus: a strain of artichoke latent virus or a new macluravirus infecting artichoke?

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Abstract

An elongated virus was isolated from artichoke crops in Liguria, and a 700-bp fragment was amplified by RT-PCR using oligonucleotides to detect members of the family Potyviridae. Comparison of fragment sequences showed 98% identity at the nucleotide level with the ranunculus isolate of the macluravirus Ranunculus latent virus (RaLV). RaLV was then detected by DAS-ELISA in symptomatic and asymptomatic artichoke plants from Liguria, Sardinia and Latium. The sequence of a 5.5-kb region was assembled from a cDNA library, and a 500-bp NIa fragment showed 80% identity to Artichoke latent virus.

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Acknowledgments

We wish to thank C. Perrone for her skilful technical assistance in the laboratory and the greenhouse.

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Authors and Affiliations

  1. Istituto di Virologia Vegetale, Sez. di Torino, CNR, Strada delle Cacce n73, Torino, 10135, Italy

    M. Ciuffo, R. Lenzi & M. Turina

  2. Dipartimento per la Ricerca nelle Produzioni Vegetali, AGRIS Sardegna, V.le Trieste 111, 09123, Cagliari, Italy

    M. Testa

Authors
  1. M. Ciuffo
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  2. M. Testa
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  3. R. Lenzi
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  4. M. Turina
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Correspondence to M. Turina.

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Ciuffo, M., Testa, M., Lenzi, R. et al. Ranunculus latent virus: a strain of artichoke latent virus or a new macluravirus infecting artichoke?. Arch Virol 156, 1053–1057 (2011). https://doi.org/10.1007/s00705-011-0949-4

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  • DOI: https://doi.org/10.1007/s00705-011-0949-4

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