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Effects of baicalein on Sendai virus in vivo are linked to serum baicalin and its inhibition of hemagglutinin-neuraminidase

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Abstract

Parainfluenza viruses are significant respiratory-tract pathogens that are notorious for infecting children. However, there are no clinical drugs to control the infection caused by these viruses. Sendai virus (SeV) belongs to the family Paramyxoviridae and causes fatal pneumonia in mice, its natural host. Baicalein is a flavonoid derived from the root of Scutellaria baicalensis, which is a traditional Chinese medicine that has been used for hundreds of years and has demonstrated a variety of biological activities. Our findings reveal that oral administration of baicalein to mice infected with Sendai virus results in a significant reduction in virus titers in the lungs and protection from death. The in vivo inhibitory effects of baicalein on Sendai virus are determined by baicalin in the serum. The mean IC50 of baicalin was 0.71 μg/ml in an HA inhibition assay and 3.22 μg/ml in an NA inhibition assay. The mean IC50 of baicalin in a CPE assay was measured to be 0.70 μg/ml, and significant inhibition was observed in a plaque assay at a concentration of 1.6 μg/ml baicalin in overlay medium, which suggests that baicalein is a potential anti-parainfluenzaviral agent in vivo.

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Acknowledgments

We would like to thank Prof. Wenyuan Liu of the Department of Pharmaceutical Analysis, China Pharmaceutical University, for excellent analytical assistance. This study was sponsored by the Qing Lan Project (2008) from Jiangsu province, the “111 Project” from the Ministry of Education of China and State Administration of Foreign Expert Affairs of China (No. 111-2-07), the Fundamental Research Funds for the Central Universities (Program No. JKY2009055), and National Fund for Fostering Talents of Basic Science(NFFTBS)J0630858.

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Authors and Affiliations

  1. School of Life Science and Technology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, China

    Jie Dou, Lili Chen, Ge Xu, Lei Zhang, Huimin Zhou, Hui Wang, Zhenzhen Su, Mengyun Ke & Changlin Zhou

  2. Department of Physiology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, China

    Qinglong Guo

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  1. Jie Dou
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  2. Lili Chen
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  3. Ge Xu
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  10. Changlin Zhou
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Correspondence to Changlin Zhou.

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Jie Dou and Lili Chen contributed equally to this work.

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Dou, J., Chen, L., Xu, G. et al. Effects of baicalein on Sendai virus in vivo are linked to serum baicalin and its inhibition of hemagglutinin-neuraminidase. Arch Virol 156, 793–801 (2011). https://doi.org/10.1007/s00705-011-0917-z

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  • DOI: https://doi.org/10.1007/s00705-011-0917-z

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