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Asialoglycoprotein receptor interacts with the preS1 domain of hepatitis B virus in vivo and in vitro

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Abstract

Background

The preS1 domain of the large envelope protein has been identified as an essential viral structure involved in hepatitis B virus (HBV) attachment. However, the cellular receptor(s) for HBV has not yet been identified.

Aims

To identify a cell-surface receptor for HBV, which could elucidate the molecular mechanism of HBV infection.

Methods

A novel yeast two-hybrid system was used to screen proteins interacting with the preS1 region of HBV. Their interaction was verified by yeast cotransformation, coimmunoprecipitation and mammalian two-hybrid assay, while their intracellular and tissue localization was analyzed by confocal microscopy and immunohistochemistry, respectively.

Results

Asialoglycoprotein receptor (ASGPR) interacted specifically and directly with the preS1 domain of HBV in vivo and in vitro. The levels of expression of preS1 and ASGPR in the liver were similar and correlated with each other.

Conclusions

ASGPR is a candidate receptor for HBV that mediates further steps of HBV entry.

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Acknowledgments

Thanks go to our colleagues in the laboratories for providing advice and help. This study was funded by the National Natural Science Foundation of China (grant 30571649).

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Authors and Affiliations

  1. Department of Infectious Diseases, The First Affiliated Hospital of Medical college of Xi’an Jiaotong University, No. 277 Yanta West Road, Xi’an 710061, Shaanxi Province, China

    Xi Zhang, Shu-mei Lin, Tian-yan Chen, Min Liu, Feng Ye, Yun-ru Chen, Lei Shi, Ying-li He, Lie-xiu Wu, Shu-qin Zheng, Ying-ren Zhao & Shu-lin Zhang

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  1. Xi Zhang
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Correspondence to Shu-mei Lin.

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Zhang, X., Lin, Sm., Chen, Ty. et al. Asialoglycoprotein receptor interacts with the preS1 domain of hepatitis B virus in vivo and in vitro . Arch Virol 156, 637–645 (2011). https://doi.org/10.1007/s00705-010-0903-x

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  • DOI: https://doi.org/10.1007/s00705-010-0903-x

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