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Improvement of the H5N1 influenza virus vaccine strain to decrease the pathogenicity in chicken embryos

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Abstract

The avian influenza vaccine strain A/duck/Hokkaido/Vac-1/2004 (H5N1) (Vac-1) was found to be pathogenic in chicken embryos (CEs). In order to decrease the pathogenicity of Vac-1 in CEs, a series of reassortant viruses was generated between Vac-1 and A/Puerto Rico/8/1934 (H1N1) (PR8), and their pathogenicity and growth potential were compared in CEs. The results indicated that either the PB1 or PA protein was responsible for the pathogenicity of Vac-1 in CEs. The HA titers of the allantoic fluids of CEs inoculated with the recombinant H5N1 viruses, of which pathogenicity was lower than that of the recombinant Vac-1 prepared by reverse genetics in CEs, were equivalent to those of CEs inoculated with the recombinant Vac-1. One of the reassortant viruses, rg-PR8-PA/Vac-1 (H5N1), in which the PA gene was replaced with the corresponding gene of PR8, yielded allantoic fluids with the same HA titer as that of Vac-1, indicating that this reassortant should be a good candidate as an improved vaccine strain.

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Acknowledgments

We are grateful to Dr. R. G. Webster, Dr. E. Hoffmann, and Dr. R. Webby, St. Jude Children’s Research Hospital, for kindly providing A/Puerto Rico/8/1934 (H1N1) and pHW72-EGFP. The present work was supported in part by the Program of Founding Research Centers for Emerging and Reemerging Infectious Disease from the Ministry of Education, Culture, Sports, Science, and Technology, Japan, and Japan Racing and Livestock Promotion Foundation. We want to thank Dr. K. Soda for providing much advice about this manuscript.

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Authors and Affiliations

  1. Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan

    Norikazu Isoda, Yoshihiro Sakoda, Masatoshi Okamatsu, Yoshimi Tsuda & Hiroshi Kida

  2. Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan

    Yoshimi Tsuda & Hiroshi Kida

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  1. Norikazu Isoda
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  2. Yoshihiro Sakoda
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  3. Masatoshi Okamatsu
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  4. Yoshimi Tsuda
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  5. Hiroshi Kida
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Correspondence to Hiroshi Kida.

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Isoda, N., Sakoda, Y., Okamatsu, M. et al. Improvement of the H5N1 influenza virus vaccine strain to decrease the pathogenicity in chicken embryos. Arch Virol 156, 557–563 (2011). https://doi.org/10.1007/s00705-010-0890-y

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  • DOI: https://doi.org/10.1007/s00705-010-0890-y

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