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Complete nucleotide sequence of capsicum chlorosis virus isolated from Phalaenopsis orchid and the prediction of the unexplored genetic information of tospoviruses

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Abstract

Phalaenopsis orchids are popular ornamentals all over the world. A tospovirus, capsicum chlorosis virus (CaCV-Ph) had been identified as the cause of chlorotic ringspots on leaves of Phalaenopsis orchids in Taiwan. The tripartite genome of CaCV-Ph was found to contain 3608, 4848 and 8916 nt of S, M and L RNAs, respectively. Phylogenetic analysis of the nucleocapsid (N) protein confirmed that CaCV-Ph is a member of the watermelon silver mottle virus (WSMoV) serogroup in the genus Tospovirus. Based on the relations among the nonstructural protein (NSs), glycoprotein (GnGc), thrips genera, host and geographical distribution, tospoviruses and thrips could be classified into two major types: WSMoV-Thrips-Asian and Tomato spotted wilt virus (TSWV)-Frankliniella-EuroAmerican. The proline (P459) of all tospoviral Gn proteins was indispensable for thrips transmission, but the RGD motif, which is maintained by only six tospoviruses, may not be required for thrips transmission. An RdRp catalytic domain found in the conserved region of the L protein may recognize the typically conserved sequences on the 5’ and 3’ terminal regions (5’ AGAGCAAU 3’).

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Acknowledgments

We are grateful to Dr. Wen-Hsiung Ko, Professor Emeritus of the University of Hawaii at Manoa and Dr. Chung-Jan Chang, Professor of the University of Georgia at Griffin for their critical review of this manuscript. We also thank Dr. Shyi-Dong Yeh for valuable discussions. This work was supported by grants (NSC94-2313-B-005-003; NSC98-2811-B-005-029) from the National Science Council, Executive Yuan, Taiwan.

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Authors and Affiliations

  1. Department of Plant Pathology, National Chung Hsing University, Taichung 402, Taiwan

    You-Xiu Zheng & Fuh-Jyh Jan

  2. Taichung District Agriculture Improvement Station, Changhua 515, Taiwan

    Ching-Chung Chen

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  1. You-Xiu Zheng
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  2. Ching-Chung Chen
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Correspondence to Fuh-Jyh Jan.

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Zheng, YX., Chen, CC. & Jan, FJ. Complete nucleotide sequence of capsicum chlorosis virus isolated from Phalaenopsis orchid and the prediction of the unexplored genetic information of tospoviruses. Arch Virol 156, 421–432 (2011). https://doi.org/10.1007/s00705-010-0874-y

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