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Determination of the complete nucleotide sequence of a lupine potyvirus isolate from the Czech Republic reveals that it belongs to a new member of the genus Potyvirus

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Abstract

The complete nucleotide sequence of the ssRNA genome of a lupine potyvirus (LP) isolate was determined. It comprised 10,113 nucleotides excluding the poly(A) tail. Phylogenetic analysis of CP protein sequences identified pepper veinal mottle virus, narcissus yellow stripe virus, and chili veinal mottle virus as the closest relatives, sharing coat protein amino acid sequence identities of only about 64% with the LP isolate. Thus, LP can be regarded as a member of a newly described potyvirus species, for which the name Lupine mosaic virus (LuMV) is proposed.

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References

  1. Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479

    Article  CAS  PubMed  Google Scholar 

  2. Berger PH, Adams MJ, Barnett OW, Brunt AA, Hammond J, Hill JH, Jordan RL, Kashiwazaki S, Rybicki E, Spence N, Stenger DC, Ohki ST, Uyeda I, van Zaayen A, Valkonen J, Vetten HJ (2005) Potyviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. VIIIth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, London, pp 819–829

  3. Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L, Zurcher EJ (eds) (1996 onwards). Plant viruses online: descriptions and lists from the VIDE database. Version: 20th August 1996. http://biology.anu.edu.au/Groups/MES/vide/

  4. Chung BYM, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. Proc Nat Acad Sci USA 105:5897–5902

    Article  CAS  PubMed  Google Scholar 

  5. Gal-On A (2000) A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptom expression in cucurbits and elicits protection against the severe homologous virus. Phytopathology 90:467–473

    Article  CAS  PubMed  Google Scholar 

  6. Gibbs AJ, Mackenzie AM, Gibbs MJ (2003) The ‘potyvirid primers’ will probably provide phylogenetically informative DNA fragments from all species of Potyviridae. J Virol Meth 112:41–44

    Article  CAS  Google Scholar 

  7. Ha C, Coombs S, Revill PA, Harding RM, Vu M, Dale JL (2008) Design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. Arch Virol 153:25–36

    Article  CAS  PubMed  Google Scholar 

  8. Koonin EV, Choi GH, Nuss DL, Shapira R, Carrington JC (1991) Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses. Proc Natl Acad Sci USA 88:10647–10651

    Article  CAS  PubMed  Google Scholar 

  9. Kurlovich BS, Stankevich AK (2002) In: Lupins geography, classification, genetic recourses and breeding. Intan Publishing House, St. Petersburg, pp 42–43

  10. Lopez-Moya JJ, Wang RY, Pirone TP (1999) Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. J Gen Virol 80:3281–3288

    CAS  PubMed  Google Scholar 

  11. Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA (1987) Selection of AUG initiation codons differs in plants and animals. EMBO J 6:43–48

    CAS  PubMed  Google Scholar 

  12. Robertson NL (2004) The biology of a new virus isolated from lupinus nootkatensis plants in Alaska. Plant Pathol 53:569–576

    Article  CAS  Google Scholar 

  13. Sarkisova T, Petrzik K (2009) A new potyvirus identified in Czech Republic. Acta Virol 53:143

    CAS  PubMed  Google Scholar 

  14. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Bio Evol 24:1596–1599

    Article  CAS  Google Scholar 

  15. Urcuqui-Inchima S, Haenni A-L, Bernardi F (2001) Potyvirus proteins: a wealth of functions. Virus Res 74:157–175

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The work was supported by Grant QH71145 of the National Agency for Agriculture Research of the Czech Republic and Grant AV0Z50510513 of the Academy of Sciences of the Czech Republic. The authors thank O. Kubešová for her excellent technical assistance.

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  1. Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic

    Tatiana Sarkisova & Karel Petrzik

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  1. Tatiana Sarkisova
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  2. Karel Petrzik
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Correspondence to Karel Petrzik.

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Sarkisova, T., Petrzik, K. Determination of the complete nucleotide sequence of a lupine potyvirus isolate from the Czech Republic reveals that it belongs to a new member of the genus Potyvirus . Arch Virol 156, 167–169 (2011). https://doi.org/10.1007/s00705-010-0843-5

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  • DOI: https://doi.org/10.1007/s00705-010-0843-5

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