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Molecular analysis of new isolates of Tomato leaf curl Philippines virus and an associated betasatellite occurring in the Philippines

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Abstract

Three new begomovirus isolates and one betasatellite were obtained from a tomato plant exhibiting leaf curl symptom in Laguna, the Philippines. Typical begomovirus DNA components representing the three isolates (PH01, PH02 and PH03) were cloned, and their full-length sequences were determined to be 2754 to 2746 nucleotides. The genome organizations of these isolates were similar to those of other Old World monopartite begomoviruses. The sequence data indicated that PH01 and PH02 were variants of strain B of the species Tomato leaf curl Philippines virus, while PH03 was a variant of strain A of the species Tomato leaf curl Philippines virus. These isolates were designated ToLCPV-B[PH:Lag1:06], ToLCPV-B[PH:Lag2:06], and ToLCPV-A[PH:Lag3:06], respectively. Phylogenetic analysis revealed that the present isolates form a separate monophyletic cluster with indigenous begomoviruses reported earlier in the Philippines. A betasatellite isolated from same sample belongs to the betasatellite species Tomato leaf curl Philippines betasatellite and designated Tomato leaf curl Philippines betasatellite-[Philippines:Laguna1:2006], ToLCPHB-[PH:Lag1:06]. When co-inoculated with this betasatellite, tomato leaf curl Philippines virus induced severe symptoms in N. benthamiana and Solanum lycopersicum plants. Using a PVX-mediated transient assay, we found that the C4 and C2 proteins of tomato leaf curl Philippines virus and the βC1 protein of ToLCPHB-[PH:Lag1:06] function as a suppressor of RNA silencing.

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References

  1. Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X, Fauquet CM (2008) Recommendation for the classification and nomenclature of the DNA-β satellites of begomoviruses. Arch Virol 153:763–781

    Article  CAS  PubMed  Google Scholar 

  2. Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID, Dhawan P, Rishi N, Siwatch SS, Abdel-Salam AM, Brown JK, Zafar Y, Markham PG (2003) Diversity of DNA β: a satellite molecule associated with some monopartite begomoviruses. Virology 312:106–121

    Article  CAS  PubMed  Google Scholar 

  3. Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG (2001) Identification of DNA components required for induction of cotton leaf curl disease. Virology 285:234–243

    Article  CAS  PubMed  Google Scholar 

  4. Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17:6739–6746

    Article  CAS  PubMed  Google Scholar 

  5. Dolores LM, Bajet NB (1995) Isolation and transmission of Tomato leaf curl virus in the Philippines. Philippine J Phytopathol 31:40–51

    Google Scholar 

  6. Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain determination and nomenclature. Arch Virol 153:783–821

    Article  CAS  PubMed  Google Scholar 

  7. Jose J, Usha R (2003) Bhendi yellow vein mosaic disease in India is caused by association of a DNAβ satellite with a begomovirus. Virology 305:310–315

    Article  CAS  PubMed  Google Scholar 

  8. Kon T, Dolores LM, Murayama A, Bajet NB, Hase S, Takahashi H, Ikegami M (2002) Genome organization of an infectious clone of Tomato leaf curl virus (Philippines), a new monopartite begomovirus. J Phytopathol 150:587–591

    Article  CAS  Google Scholar 

  9. Kon T, Hidayat SH, Hase S, Takahashi H, Ikegami M (2006) The natural occurrence of two distinct begomoviruses associated with DNAβ and a recombinant DNA in a tomato plant from Indonesia. Phytopathology 96:517–525

    Article  CAS  PubMed  Google Scholar 

  10. Kon T, Sharma P, Ikegami M (2007) Suppressor of RNA silencing encoded by the monopartite tomato leaf curl Java begomovirus. Arch Virol 152:1273–1282

    Article  CAS  PubMed  Google Scholar 

  11. Mansoor S, Briddon RW, Zafar Y, Stanley J (2003) Geminivirus disease complexes: an emerging threat. Trends Plant Sci 8:128–134

    Article  CAS  PubMed  Google Scholar 

  12. Matsuda N, Sharma P, Bajet NB, Ikegami M (2008) Molecular characterization of a new strain of Tomato leaf curl Philippines virus and its associated satellite DNAβ molecule: further evidence for natural recombination amongst begomoviruses. Arch Virol 153:961–967

    Article  CAS  PubMed  Google Scholar 

  13. Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347

    Article  CAS  Google Scholar 

  14. Saunders K, Bedford ID, Briddon RW, Markham PG, Wong SM, Stanley J (2000) A unique virus complex causes Ageratum yellow vein disease. Proc Natl Acad Sci USA 97:6890–6895

    Article  CAS  PubMed  Google Scholar 

  15. Saunders K, Salim N, Mali VR, Malathi VG, Briddon RW, Stanley J (2002) Characterization of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: evidence for acquisition of a DNA B component by a monopartite begomovirus. Virology 293:63–74

    Article  CAS  PubMed  Google Scholar 

  16. Sharma P, Ikegami M (2008) RNA silencing suppressors of geminiviruses. J Gen Plant Pathol 73:189–202

    Article  Google Scholar 

  17. Sharma P, Ikegami M (2010) Tomato leaf curl Java virus V2 protein determines the virulence, hypersensitive response and post transcriptional gene silencing. Virology 396:85–93

    Article  CAS  PubMed  Google Scholar 

  18. Vanitharani R, Chellappan P, Facquet CM (2005) RNA silencing and geminiviruses. Trends Plant Sci 10:1360–1385

    Google Scholar 

  19. Voinnet O, Pinto YM, Baulcombe DC (1999) Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc Natl Acad Sci USA 96:14147–14152

    Article  CAS  PubMed  Google Scholar 

  20. Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142:145–164

    Article  CAS  Google Scholar 

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Acknowledgments

We are thankful to David Baulcombe for the PVX vector and N. benthamiana 16c seeds. We also thank Project Director, DWR and Dr. N. Rishi for their helpful suggestions during preparation of manuscript. This work was partly supported by grants-in-aid from the Academic Frontier Promotion Program from the Ministry of Education, Culture, Sport, Science and Technology of Japan to M.I. The first author is grateful to the Japan Society for Promotion of Science for financial assistance under the Young Scientist Grant-in-Aid scheme.

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Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan

    Pradeep Sharma, N. Matsuda & M. Ikegami

  2. Department of Plant Pathology, Faculty of Agriculture, University of the Philippines, Los Banos, Philippines

    N. B. Bajet

  3. Division of Crop Improvement, Directorate of Wheat Research, Karnal, India

    Pradeep Sharma

Authors
  1. Pradeep Sharma
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  2. N. Matsuda
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  3. N. B. Bajet
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  4. M. Ikegami
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Corresponding author

Correspondence to Pradeep Sharma.

Additional information

Nucleotide sequence data are available in GenBank under accession numbers AB377111 (PH01), AB37712 (PH02), AB377113 (PH03) and AB308071 (PH0β).

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Sharma, P., Matsuda, N., Bajet, N.B. et al. Molecular analysis of new isolates of Tomato leaf curl Philippines virus and an associated betasatellite occurring in the Philippines. Arch Virol 156, 305–312 (2011). https://doi.org/10.1007/s00705-010-0837-3

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  • DOI: https://doi.org/10.1007/s00705-010-0837-3

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