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A single amino acid change in HC-Pro of soybean mosaic virus alters symptom expression in a soybean cultivar carrying Rsv1 and Rsv3

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Abstract

It is generally believed that infidelity of RNA virus replication combined with R-gene-driven selection is one of the major evolutionary forces in overcoming host resistance. In this study, we utilized an avirulent soybean mosaic virus (SMV) mutant to examine the possibility of emergence of mutant viruses capable of overcoming R-gene-mediated resistance during serial passages. Interestingly, we found that the emerged progeny virus induced severe rugosity and local necrotic lesions in Jinpumkong-2 (Rsv1 + Rsv3) plants, while SMV-G7H provoked a lethal systemic hypersensitive response. Genome sequence analysis of the emerged progeny virus revealed that the mutation in CI that had caused SMV-G7H to lose its virulence was restored to the original sequence, and a single amino acid was newly introduced into HC-Pro, which means that the symptom alteration was due to this single amino acid mutation in HC-Pro. Our results suggest that SMV HC-Pro functions as a symptom determinant in the SMV-soybean pathosystem.

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Acknowledgments

This research was supported in part by grants from the Rural Development Administration (nos. 20080401-034-010-009-02-00 & 20090101-060-029-001-03-00) and the Korea Institute of Planning and Evaluation for Technology of Food, Agriculture, Forestry and Fisheries (04601-20090004). JKS was supported by a graduate research fellowship from the Ministry of Education, Science and Technology through the Brain Korea 21 Project.

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  1. Jang-Kyun Seo

    Present address: Department of Plant Pathology and Microbiology, University of California, Riverside, CA, 92521, USA

Authors and Affiliations

  1. Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Jang-Kyun Seo & Kook-Hyung Kim

  2. National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Republic of Korea

    Seong-Han Sohn

  3. Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea

    Kook-Hyung Kim

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  1. Jang-Kyun Seo
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Correspondence to Kook-Hyung Kim.

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Seo, JK., Sohn, SH. & Kim, KH. A single amino acid change in HC-Pro of soybean mosaic virus alters symptom expression in a soybean cultivar carrying Rsv1 and Rsv3 . Arch Virol 156, 135–141 (2011). https://doi.org/10.1007/s00705-010-0829-3

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  • DOI: https://doi.org/10.1007/s00705-010-0829-3

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