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Description of a new putative virus infecting the conifer pathogenic fungus Heterobasidion parviporum with resemblance to Heterobasidion annosum P-type partitivirus

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Abstract

The complete sequences of two double-stranded RNA segments from the fungus Heterobasidion parviporum were characterized. The larger segment (2,290 bp) contained an open reading frame encoding a putative RNA-dependent RNA polymerase (RdRp, 722 aa), while the smaller one (2,238 bp) encoded a putative coat protein of 659 aa. Based on phylogenetic analysis, the dsRNA segments constitute the genome of a new virus assigned to the family Partitiviridae and named Heterobasidion RNA virus 2 (HetRV2). The RdRp segment was clearly related to H. annosum P-type partitivirus (aa similarity of 59%) but was only distantly related to previously described viruses of H. parviporum (aa similarity 26–35%). The dsRNA could be experimentally transmitted to all five species of the Heterobasidion annosum sensu lato complex and two species of the H. insulare complex, indicating that horizontal transfer between these intersterile fungal species is possible.

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Acknowledgments

This study was financially supported by the Academy of Finland (Project 122565) and the Finnish Forest Research Institute. We thank Ms Marja-Leena Santanen, Ms Sonja Savin and Ms Sonja Sarsila for skillful technical assistance. The collectors of the Heterobasidion strains used in the transmission experiments (Y.C. Dai, T. Dogmus-Lehtijärvi, D. Goheen, T. Kirisits, K. Korhonen, A. Lehtijärvi, E. Motta, T. Piri and T. Yamaguchi) are also gratefully acknowledged.

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Authors and Affiliations

  1. Finnish Forest Research Institute, Jokiniemenkuja 1, P.O. Box 18, 01301, Vantaa, Finland

    Eeva J. Vainio & Jarkko Hantula

  2. Department of Forest Sciences, Faculty of Agriculture and Forestry, University of Helsinki, Latokartanonkaari 7, P.O. Box 27, 00014, Helsinki, Finland

    Susanna Keriö

Authors
  1. Eeva J. Vainio
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  2. Susanna Keriö
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  3. Jarkko Hantula
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Correspondence to Eeva J. Vainio.

Additional information

Nucleotide sequence data reported are available in the GenBank database under accession numbers HM565953 and HM565954.

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Vainio, E.J., Keriö, S. & Hantula, J. Description of a new putative virus infecting the conifer pathogenic fungus Heterobasidion parviporum with resemblance to Heterobasidion annosum P-type partitivirus. Arch Virol 156, 79–86 (2011). https://doi.org/10.1007/s00705-010-0823-9

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  • DOI: https://doi.org/10.1007/s00705-010-0823-9

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