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Detection of infectious myonecrosis virus in penaeid shrimps using immunoassays: usefulness of monoclonal antibodies directed to the viral major capsid protein

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Abstract

Despite the economic impact of the infectious myonecrosis virus (IMNV) on shrimp farms in several countries, no method for immunological detection is currently available. With the aim of developing immunodiagnostic methods for IMNV detection in infected shrimps, a recombinant fragment of the IMNV major capsid protein gene encoding amino acids 105–297 (rIMNV105–297) was heterologously expressed in Escherichia coli and used to immunize Balb/c mice, generating monoclonal antibodies (MAbs). Six hybridomas were obtained, and four of these recognized the presence of IMNV in tissue homogenates from naturally infected shrimps by immunodot blot assay. Among these MAbs, three were able to detect a ~100-kDa protein, which corresponds to the predicted mass of the IMNV major capsid protein, as well as viral inclusion bodies in muscle fibroses by western blot and immunohistochemistry. Two MAbs showed high specificity and sensitivity, showing no cross-reaction with healthy shrimp tissues in any assays, indicating their usefulness for IMNV detection.

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Acknowledgments

This work was supported by grants from International Foundation for Science (IFS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Ministério da Agricultura, Pecuária e Abastecimento (MAPA), and Financiadora de Estudos e Projetos (FINEP).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC, 88040-970, Brazil

    Mariana Borsa, Caroline H. Seibert, Rafael D. Rosa, Patrícia H. Stoco, Edmundo C. Grisard, Carlos R. Zanetti & Aguinaldo R. Pinto

  2. Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC, 88040-970, Brazil

    Eduardo Cargnin-Ferreira

  3. Laboratório de Patologia de Organismos Aquáticos, Embrapa Meio-Norte, BR 343, km 35, Parnaíba, PI, 64200-970, Brazil

    Alitiene M. L. Pereira

Authors
  1. Mariana Borsa
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  2. Caroline H. Seibert
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  3. Rafael D. Rosa
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  4. Patrícia H. Stoco
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  5. Eduardo Cargnin-Ferreira
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  6. Alitiene M. L. Pereira
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  7. Edmundo C. Grisard
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  8. Carlos R. Zanetti
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  9. Aguinaldo R. Pinto
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Corresponding author

Correspondence to Aguinaldo R. Pinto.

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Borsa, M., Seibert, C.H., Rosa, R.D. et al. Detection of infectious myonecrosis virus in penaeid shrimps using immunoassays: usefulness of monoclonal antibodies directed to the viral major capsid protein. Arch Virol 156, 9–16 (2011). https://doi.org/10.1007/s00705-010-0810-1

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  • DOI: https://doi.org/10.1007/s00705-010-0810-1

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