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Hepatitis B virus infection and replication in primary cultured human granulosa cells

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Abstract

To investigate the infection and replication of hepatitis B virus (HBV) in primary cultured human granulosa cells. Human granulosa cells were cultured with HBV-positive serum. Media were collected and assayed for HBsAg and HBeAg by ELISA, and HBV DNA by quantitative PCR. HBsAg and HBcAg were detected by immunocytochemistry in cultured cells. HBV DNA and RNA were extracted and amplified by nested PCR. Intracellular HBV DNA was localized by in situ hybridization. By co-cultivation of human GCs with HBV-positive serum, a system was established to study HBV infection and replication in GCs. HBsAg in medium could be detected from 4 to 96 h, and HBV DNA could be detected from 12 to 96 h after exposure. HBsAg and HBcAg showed positive signals by immunocytochemistry. A 206-bp fragment was amplified by nested PCR to detect HBV DNA and RNA in granulosa cells. HBV DNA was detected in GC nuclei by in situ hybridization. HBV can infect and replicate in human primary granulosa cells. This culture system could enable us to study infection of ova by HBV.

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Acknowledgment

Supported by the National Natural Science Foundation of China, No. 30371277

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Authors and Affiliations

  1. Department of Infectious Disease, The First Hospital of Xi’an Jiaotong University, Jiankang Road 1#, Xi’an, 710061, Shaanxi, China

    Yan Jin, Feng Ye, Yingren Zhao, Shumei Lin, Tianyan Chen, Min Liu, Yingli He & Shulin Zhang

  2. Reproductive Center, Shaanxi Maternal and Child Care Service Hospital, Xi’an, China

    Juanzi Shi & Hongtao Qiu

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  1. Yan Jin
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  2. Feng Ye
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  3. Juanzi Shi
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  7. Tianyan Chen
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  8. Min Liu
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  9. Yingli He
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  10. Shulin Zhang
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Correspondence to Feng Ye.

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Jin, Y., Ye, F., Shi, J. et al. Hepatitis B virus infection and replication in primary cultured human granulosa cells. Arch Virol 156, 1–7 (2011). https://doi.org/10.1007/s00705-010-0808-8

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  • DOI: https://doi.org/10.1007/s00705-010-0808-8

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