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Analysis of complete genomic sequences of isolates of the Sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains

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Abstract

The complete nucleotide sequence of the isolate C1 of Sweet potato feathery mottle virus (SPFMV) strain C and the 5′ region of several other strains were determined and analyzed together with the sequences of isolates representing the EA, RC and O strains. This provided molecular evidence for the reclassification of SPFMV strains into two species and the occurrence of a complex recombinant isolate. Analysis also revealed a hypervariable domain in the P1 protein, which separates an N-terminal region unique to SPFMV and members of the ipomovirus species Sweet potato mild mottle virus from the C-terminal protease domain, which is conserved among all potyviruses.

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Acknowledgments

Financial support from the Belgium Directorate General for Development Cooperation and the Howard Buffet Foundation is gratefully acknowledged. We thank Segundo Fuentes for providing information regarding the C1 strain and suggestion of a new virus name, and Jean Ndirigue and Michel Twizeye for sharing SPFMV-infected sweet potato samples from Rwanda and Burundi, respectively.

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Authors and Affiliations

  1. Applied Biotechnology Laboratory, International Potato Center (CIP), Apartado 1558, Lima 12, Peru

    Milton Untiveros, Dora Quispe & Jan Kreuze

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  1. Milton Untiveros
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  2. Dora Quispe
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  3. Jan Kreuze
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Corresponding author

Correspondence to Jan Kreuze.

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M. Untiveros and D. Quispe have contributed equally to this work.

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Untiveros, M., Quispe, D. & Kreuze, J. Analysis of complete genomic sequences of isolates of the Sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains. Arch Virol 155, 2059–2063 (2010). https://doi.org/10.1007/s00705-010-0805-y

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  • DOI: https://doi.org/10.1007/s00705-010-0805-y

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