Abstract
Eight bluetongue viruses (BTV) were isolated in BHK-21 cell culture from blood of goats suffering from peste des petits ruminants. These viruses were identified as BTV serotype 1 (BTV-1) by RT-PCR using VP2-gene-based primers coupled with sequencing of the PCR products. All of the isolates showed similar genome migration profile in 8% polyacrylamide gel electrophoresis. The genome segment-2 (seg-2) of one isolate (MKD18/India/2008) was amplified piecemeal by overlapping PCR, and the products were sequenced to obtain full-length seg-2. Phylogenetic analysis based on the seg-2 sequence revealed that MKD18 is closely related to Australian BTV-1 isolates, with 86.3–86.8% nucleotide identity. Phylogenetic analysis based on the partial sequence of seg-2 (541 bp, nucleotides 1,304–1,844) showed that the Indian BTV-1 isolates, namely, MKD18, Avikanagar, Sirsa-3 and Chennai, are very closely related to each other, with more than 99.6% nucleotide identity. Although a high degree of similarity exists, the Indian BTV-1 isolates collected over the past 25 years should be studied to demonstrate the co-existence of different VP2 antigenic profiles.
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Acknowledgments
This work has been supported by an ICAR project–All India Network Programme on Bluetongue. BTV serotyping was done at the Department of Animal Biotechnology, CCS Haryana Agricultural University, and the nucleotide sequencing was carried out at the Central FMDV Typing Lab, PD-FMD, Mukteswar. We thank the Head, Division of Virology, IVRI for support.
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Biswas, S.K., Chand, K., De, A. et al. Isolation of bluetongue virus serotype 1 (BTV-1) from goats and its phylogenetic relationship to other BTV-1 isolates worldwide based on full-length sequence of genome segment-2. Arch Virol 155, 2041–2046 (2010). https://doi.org/10.1007/s00705-010-0794-x
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DOI: https://doi.org/10.1007/s00705-010-0794-x