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Molecular variation of hop mosaic virus isolates

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Abstract

Hop mosaic virus (HpMV), a member of the genus Carlavirus, is importance to hop production worldwide. We identified variation in nucleic and amino acid sequences among 23 HpMV isolates from Australia, the USA, the Czech Republic, South Africa and Japan using a 1,455-bp fragment covering the 3′ end of the virus genome including ORFs 4, 5 and 6. Three clusters of two or more isolates were identified in phylogenies of the total nucleotide sequence and the coat protein (ORF5) amino acid sequence. Two of these clusters combined in analyses of ORF4 and ORF6 amino acid sequences. Isolates from within and outside of Australia were found in each cluster, indicating that sequence variation was not associated with geographic source. Monitoring of HpMV variants in the field and evaluation of the impact of variants on vector association, rate of spread, and hop yield and quality can now be undertaken.

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Acknowledgments

We thank Hop Products Australia Pty Ltd for access to hop gardens for isolate collection. HpMV isolates were kindly provided by Dr. K. Eastwell, E. Jooste and P. Svoboda. We also thank Drs. S. Radisek, U. Skomra, R. Beatson and D. Barbara for providing hop material for testing and Dr. S. Pethybridge and P. Cross for advice on ELISA protocols.

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Authors and Affiliations

  1. Tasmanian Institute of Agricultural Research, University of Tasmania, 13 St Johns Ave, New Town, TAS, 7008, Australia

    Fiona S. Poke, Damian R. Crowle & Calum R. Wilson

  2. Menzies Research Institute, University of Tasmania, Private Bag 23, Hobart, TAS, 7000, Australia

    Fiona S. Poke

  3. School of Plant Science, University of Tasmania, Private Bag 55, Hobart, TAS, 7001, Australia

    Simon P. Whittock

  4. Hop Products Australia, 26 Cambridge Rd, Bellerive, TAS, 7018, Australia

    Simon P. Whittock

Authors
  1. Fiona S. Poke
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  2. Damian R. Crowle
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  3. Simon P. Whittock
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  4. Calum R. Wilson
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Corresponding author

Correspondence to Calum R. Wilson.

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Poke, F.S., Crowle, D.R., Whittock, S.P. et al. Molecular variation of hop mosaic virus isolates. Arch Virol 155, 1721–1724 (2010). https://doi.org/10.1007/s00705-010-0780-3

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  • DOI: https://doi.org/10.1007/s00705-010-0780-3

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