Archives of Virology

, Volume 155, Issue 9, pp 1529–1534 | Cite as

A novel species of mastrevirus (family Geminiviridae) isolated from Digitaria didactyla grass from Australia

  • Rob W. Briddon
  • Darren P. Martin
  • Betty E. Owor
  • Lara Donaldson
  • Peter G. Markham
  • Ray S. Greber
  • Arvind Varsani
Annotated Sequence Record

Abstract

Mastreviruses (family Geminiviridae) that infect monocotyledonous plants occur throughout the temperate and tropical regions of Asia, Africa, Europe and Australia. Despite the identification of a very diverse array of mastrevirus species whose members infect African monocots, few such species have been discovered in other parts of the world. For example, the sequence of only a single monocot-infecting mastrevirus, Chloris striate mosaic virus (CSMV), has been reported so far from Australia, even though earlier biological and serological studies suggested that other distinct mastreviruses were present. Here, we have obtained the complete nucleotide sequence of a virus from the grass Digitaria didactyla originating from Australia. Analysis of the sequence shows the virus to be a typical mastrevirus, with four open reading frames, two in each orientation, separated by two non-coding intergenic regions. Although it showed the highest levels of sequence identity to CSMV (68.7%), their sequences are sufficiently diverse for the virus to be considered a member of a new species in the genus Mastrevirus, based on the present species demarcation criteria. We propose that the name first used during the 1980s be used for this species, Digitaria didactyla striate mosaic virus (DDSMV).

Keywords

Streak Virus Wheat Dwarf Virus Species Demarcation Criterion Tobacco Yellow Dwarf Virus Dactyloctenium Aegyptium 

Notes

Acknowledgments

This study was partly supported by the South African National Foundation of Science. R.W.B. is supported by the Higher Education Commission (HEC), Government of Pakistan, under the “Foreign Faculty Hiring Program”. A.V. was supported by the Carnegie Corporation of New York. We thank Dr. Dionne Shepherd for comments and suggestions on the MS.

Supplementary material

705_2010_759_MOESM1_ESM.doc (34 kb)
Supplementary Figure 1: Genome sequence annotation of DDSMV and CSMV. Gaps introduced to optimise the alignment are indicated with a “-” character (DOC 33 kb)
705_2010_759_MOESM2_ESM.doc (24 kb)
Supplementary Figure 2: Annotated predicted movement protein amino acid sequences of DDSMV and CSMV. Gaps introduced to optimise the alignment are indicated with a “-” character (DOC 24 kb)
705_2010_759_MOESM3_ESM.doc (26 kb)
Supplementary Figure 3: Annotated predicted coat protein amino acid sequences of DDSMV and CSMV. Gaps introduced to optimise the alignment are indicated with a “-” character (DOC 25 kb)
705_2010_759_MOESM4_ESM.doc (30 kb)
Supplementary Figure 4: Annotated predicted replication-associated protein amino acid sequences of DDSMV and CSMV. Gaps introduced to optimise the alignment are indicated with a “-” character (DOC 29 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Rob W. Briddon
    • 1
  • Darren P. Martin
    • 2
  • Betty E. Owor
    • 3
  • Lara Donaldson
    • 4
  • Peter G. Markham
    • 5
  • Ray S. Greber
    • 6
  • Arvind Varsani
    • 7
    • 8
  1. 1.National Institute for Biotechnology and Genetic EngineeringFaisalabadPakistan
  2. 2.Institute of Infectious Diseases and Molecular MedicineUniversity of Cape TownObservatorySouth Africa
  3. 3.Department of Plant SciencesUniversity of CambridgeCambridgeUK
  4. 4.Department of Molecular and Cell BiologyUniversity of Cape TownRondeboschSouth Africa
  5. 5.Department of Disease and Stress BiologyJohn Innes CentreNorwichUK
  6. 6.Queensland Department of Primary IndustriesMaroochy Horticulture Research StationNambourAustralia
  7. 7.Electron Microscope UnitUniversity of Cape TownRondeboschSouth Africa
  8. 8.School of Biological SciencesUniversity of CanterburyChristchurchNew Zealand

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