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Molecular characterization of adenovirus detected from fecal samples obtained from children in the Central West region of Brazil

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Abstract

The adenoviruses are frequently associated with sporadic gastroenteritis outbreaks in different parts of the world. This study aimed at the molecular characterization of human adenoviruses (HAdV) species and serotypes, in fecal samples from children, by multiplex-PCR and by PCR–RFLP, respectively, followed by genomic sequencing. Of 39 adenovirus-positive samples, 30 (76.9%) were classified as species F, six (15.4%) as species C, and two (5.1%) as species A, and one (2.6%) had a mixed F/C pattern. The serotyping showed that 14 (41.2%) were HAdV-41, 15 (44.1%) were HAdV-40, five (14.7%) were HAdV-5, and five samples could not be serotyped. This is the first study to molecularly characterize HAdV in the Central West region of Brazil, and the results highlight the circulation of the HAdV-5 among children with acute gastroenteritis in this region.

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Acknowledgments

The authors thank the National Counsel of Technological and Scientific Development (CNPq) of Brazil for providing financial support.

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Authors and Affiliations

  1. Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua Delenda Rezende de Melo s/n, Setor Universitário, Goiânia, Goiâs, 74605-050, Brazil

    Erika Regina L. de Freitas, Ana Maria T. Borges, Fabíola S. Fiaccadori, Menira B. L. D. e Souza & Divina das Dôres P. Cardoso

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  1. Erika Regina L. de Freitas
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  2. Ana Maria T. Borges
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  3. Fabíola S. Fiaccadori
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  4. Menira B. L. D. e Souza
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  5. Divina das Dôres P. Cardoso
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Correspondence to Erika Regina L. de Freitas.

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de Freitas, E.R.L., Borges, A.M.T., Fiaccadori, F.S. et al. Molecular characterization of adenovirus detected from fecal samples obtained from children in the Central West region of Brazil. Arch Virol 155, 1693–1696 (2010). https://doi.org/10.1007/s00705-010-0748-3

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  • DOI: https://doi.org/10.1007/s00705-010-0748-3

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