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Characterization of a filamentous virus from Bermuda grass and its molecular, serological and biological comparison with Spartina mottle virus

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Abstract

Bermuda grass with mosaic symptoms have been found in many parts of Iran. No serological correlation was observed between two isolates of this filamentous virus and any of the members of the family Potyviridae that were tested. Aphid transmission was demonstrated at low efficiency for isolates of this virus, whereas no transmission through seed was observed. A DNA fragment corresponding to the 3′ end of the viral genome of these two isolates from Iran and one isolate from Italy was amplified and sequenced. A BLAST search showed that these isolates are more closely related to Spartina mottle virus (SpMV) than to any other virus in the family Potyviridae. Specific serological assays confirmed the phylogenetic analysis. Sequence and phylogenetic analysis suggested that these isolates could be considered as divergent strains of SpMV in the proposed genus Sparmovirus.

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Acknowledgments

We are grateful to Dr. W. Huth, Dr. R. Koenig and Dr. K. Richert-Pöggeler (Julius Kühn Institute, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Braunschweig, Germnay) and to Dr. Wulf Menzel (DSMZ plant virus collection, Braunschweig, Germany) for providing SpMV antiserum and isolates. We are also grateful to Dr. P. Caciagli (IVV, Istituto di Virologia Vegetale, CNR, Torino, Italy) for R. padi aphid species.

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Authors and Affiliations

  1. Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Tehran, Iran

    A. Hosseini, M. Koohi Habibi & G. H. Mosahebi

  2. Department of Plant Protection, College of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran

    A. Hosseini

  3. Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran

    K. Izadpanah

  4. DiSTA, Patologia Vegetale, Università di Bologna, Viale G. Fanin, 40-40127, Bologna, Italy

    C. Rubies-Autonell & C. Ratti

Authors
  1. A. Hosseini
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  2. M. Koohi Habibi
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  3. K. Izadpanah
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  4. G. H. Mosahebi
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  5. C. Rubies-Autonell
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Correspondence to A. Hosseini.

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Hosseini, A., Koohi Habibi, M., Izadpanah, K. et al. Characterization of a filamentous virus from Bermuda grass and its molecular, serological and biological comparison with Spartina mottle virus. Arch Virol 155, 1675–1680 (2010). https://doi.org/10.1007/s00705-010-0745-6

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  • DOI: https://doi.org/10.1007/s00705-010-0745-6

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