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Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein

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Abstract

Three anti-rabies virus (RABV) nucleoprotein (N) monoclonal antibodies (Mab) were characterized by immunofluorescence assays, western blotting, and immunohistochemistry. One of these Mabs recognized the antigen by all of the assays, while the other two recognized N only in the native form in the immunofluorescence assay. These data, together with epitope mapping studies, suggest that two anti-N Mabs recognize conformational epitopes located within the N-terminal region of the RABV N protein. The availability of Mabs specific for both linear and epitope-specific antibodies should prove valuable for rabies diagnosis as well as for RABV N protein structure–function studies.

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Acknowledgments

This work is supported partially by Public Health Service grant AI-051560 from the National Institute of Allergy and Infectious Diseases.

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Authors and Affiliations

  1. Department of Pathology, College of Veterinary Medicine, University of Georgia, 501 D.W. Brooks Drive, Athens, GA, 30602, USA

    Yan Jiang, Ying He & Zhen F. Fu

  2. Department of Pharmacological Sciences, School of Traditional Chinese Medicine, Southwest University, Chongqing, 400716, China

    Yan Jiang & Yonghuang Luo

  3. Department of Anatomy and Radiology, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA

    Frank Michel & Robert J. Hogan

  4. Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, 30602, USA

    Robert J. Hogan & Zhen F. Fu

Authors
  1. Yan Jiang
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  2. Yonghuang Luo
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  3. Frank Michel
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  4. Robert J. Hogan
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  5. Ying He
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  6. Zhen F. Fu
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Correspondence to Zhen F. Fu.

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Jiang, Y., Luo, Y., Michel, F. et al. Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein. Arch Virol 155, 1187–1192 (2010). https://doi.org/10.1007/s00705-010-0709-x

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  • DOI: https://doi.org/10.1007/s00705-010-0709-x

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