Abstract
A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
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This study was supported by the National Natural Science Foundation of China (ID: 30760181) and the Project by the National Science & Technology Pillar Program of China (ID: 2006BAD04A09).
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Ma, W., Wang, F., Yu, L. et al. Identification of an intergenic region that is not essential for replication of goatpox virus. Arch Virol 155, 1337–1341 (2010). https://doi.org/10.1007/s00705-010-0705-1
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DOI: https://doi.org/10.1007/s00705-010-0705-1