Abstract
Here, we show that heat shock cognate protein 70 (Hsc70) in shrimp cells can inhibit apoptosis induced by white spot syndrome virus (WSSV) infection. Caspase-3 protease activity of hemocytes increased significantly, correlating with a reduction in endogenous Hsc70 late in WSSV infection. Hsc70 dsRNA caused a significant increase in caspase-3 activity in the hemocytes of non-infected shrimp and WSSV-infected shrimp. We propose that upregulation of Hsc70 expression early in WSSV infection may also be used to prevent premature apoptotic cell death, and the precipitous downregulation of Hsc70 late in WSSV infection may lead to the timed induction of apoptosis.
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This work was supported by grants from the National Nature Science Foundation of China (30800852, 30972276) and the National 863 Project of China (2006AA09Z445).
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Feng Yan and Ding Xia contributed equally to the article.
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Yan, F., Xia, D., Hu, J. et al. Heat shock cognate protein 70 gene is required for prevention of apoptosis induced by WSSV infection. Arch Virol 155, 1077–1083 (2010). https://doi.org/10.1007/s00705-010-0686-0
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DOI: https://doi.org/10.1007/s00705-010-0686-0