Skip to main content
SpringerLink
Log in

The complete genome sequence of freesia mosaic virus and its relationship to other potyviruses

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

We have completed the genomic sequence of a potyvirus, freesia mosaic virus (FreMV), and compared it to those of other known potyviruses. The full-length genome sequence of FreMV consists of 9,489 nucleotides. The large protein contains 3,077 amino acids, with an AUG start codon and UAA stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of FreMV-Kr gives rise to eleven proteins (P1, HC-pro, P3, PIPO, 6K1, CI, 6K2, VPg, NIa, NIb and CP), and putative cleavage sites of each protein were identified by sequence comparison to those of other known potyviruses. Phylogenetic analysis of the polyprotein revealed that FreMV-Kr was most closely related to PeMoV and was related to BtMV, BaRMV and PeLMV, which belong to the BCMV subgroup. This is the first information on the complete genome structure of FreMV, and the sequence information clearly supports the status of FreMV as a member of a distinct species in the genus Potyvirus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Brunt AA (1995) Freesia. In: Loebenstein G, Lawson RH, Brunt AA (eds) Virus and virus-like diseases of bulb and flower crops. Wiley, Chichester, pp 274–280

    Google Scholar 

  2. Chung BYW, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. Proc Natl Acad Sci USA 105:5897–5902

    Article  CAS  PubMed  Google Scholar 

  3. Dombrovsky A, Huet H, Chejanovsky N, Raccah B (2005) Aphid transmission of a potyvirus depends on suitability of the helper component and the N terminus of the coat protein. Arch Virol 150:287–298

    Article  CAS  PubMed  Google Scholar 

  4. Flasinski S, Cassidy BG (1998) Potyvirus aphid transmission requires helper component and homologous coat protein for maximal efficiency. Arch Virol 143:2159–2172

    Article  CAS  PubMed  Google Scholar 

  5. Guaragna MA, Ndum O, Jordan RL (2006) Detection and characterization of two previously undescribed potyviruses in the terrestrial orchid Spiranthes cernua. Acta Hortic 722:209–218

    CAS  Google Scholar 

  6. Kumar Y, Hallan V, Zaidi AA (2009) First finding of Freesia mosaic virus infecting freesia in India. Plant Pathol 58:404

    Article  Google Scholar 

  7. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Article  CAS  PubMed  Google Scholar 

  8. Urcuqui-Inchima S, Haenni AL, Bernardi F (2001) Potyvirus proteins: a wealth of functions. Virus Res 74:157–175

    Article  CAS  PubMed  Google Scholar 

  9. Van Koot Y, van Slogteren DHM, Cremer MC, Camfferman J (1954) Virus verschijn-selen in Freesia’s. Tijdschr Plziekt 60:157

    Article  Google Scholar 

  10. Yu XQ, Lan YF, Wang HY, Liu JL, Zhu XP, Jari PTV, Li XD (2007) The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses. Virus Genes 35:801–806

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by an Agenda grant (200901010600310010200) and a Biogreen 21 grant (200703010340190090400) from the RDA in Korea, and in part by a grant (2010-0001460/R0602545) from the Plant Signaling Network Center of Korea University from the KNRF in Korea.

Author information

Authors and Affiliations

  1. Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women’s University, Seoul, 139-774, Korea

    H. I. Choi, H. R. Lim, Y. S. Song, M. J. Kim, S. H. Choi, Y. S. Song & K. H. Ryu

  2. National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea

    S. C. Bae

Authors
  1. H. I. Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. R. Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. S. Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. J. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. H. Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Y. S. Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. C. Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. K. H. Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. H. Ryu.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 20 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, H.I., Lim, H.R., Song, Y.S. et al. The complete genome sequence of freesia mosaic virus and its relationship to other potyviruses. Arch Virol 155, 1183–1185 (2010). https://doi.org/10.1007/s00705-010-0669-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-010-0669-1

Keywords

Search

Navigation