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Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico

Abstract

The characterization of viruses infecting wild plants is a key step towards understanding the ecology of plant viruses. In this work, the complete genomic nucleotide sequence of a new tymovirus species infecting chiltepin, the wild ancestor of Capsicum annuum pepper crops, in Mexico was determined, and its host range has been explored. The genome of 6,517 nucleotides has the three open reading frames described for tymoviruses, putatively encoding an RNA-dependent RNA polymerase, a movement protein and a coat protein. The 5′ and 3′ untranslated regions have structures with typical signatures of the tymoviruses. Phylogenetic analyses revealed that this new virus is closely related to the other tymoviruses isolated from solanaceous plants. Its host range is mainly limited to solanaceous species, which notably include cultivated Capsicum species. In the latter, infection resulted in a severe reduction of growth, indicating the potential of this virus to be a significant crop pathogen. The name of chiltepin yellow mosaic virus (ChiYMV) is proposed for this new tymovirus.

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Acknowledgments

This work was in part funded by grants “Interacciones entre virus y sus plantas huésped en poblaciones silvestres de tabaco y pimiento” (Programa de Cooperación Científica con Iberoamérica 1999, Ministerio de Educación y Cultura, Spain) and “Impacto de los patógenos en la conservación de especies amenazadas: aplicación a las poblaciones silvestres de chiltepín en México” (BIOCON05/101, Fundación BBVA, Spain).

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Correspondence to Fernando García-Arenal.

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I. Pagán and M. Betancourt contributed equally to this work.

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Supplementary Fig. S1.

Predicted secondary structure of the 5′- and 3′-untranslated regions (UTR) of the Tula 20.5 and Tula 20.8 genomic RNA. A. Secondary structure of the 5′-UTR. The three hairpins present in this region (HP 1, HP 2, and HP 3) are indicated. The overlapping protein (OP) start codon is boxed. B. Secondary structure of the 3′-UTR. The tRNA-like structure and the three upstream stem-loops are indicated. The coat protein (CP) stop codon, and the anticodon for valine are highlighted in boxes. Secondary structures were obtained using UNAfold software (http://www.bioinfo.rpi.edu/applications/hybrid/download.php) (TIFF 66 kb)

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Supplementary material 3 (DOC 38 kb)

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Pagán, I., Betancourt, M., de Miguel, J. et al. Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico. Arch Virol 155, 675–684 (2010). https://doi.org/10.1007/s00705-010-0639-7

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Keywords

  • Coat Protein
  • Pepper Plant
  • Chilli Pepper
  • Yellow Mosaic Virus
  • Capsicum Species