A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas
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Jatropha curcas mosaic disease is a newly emerging disease that challenges the productivity of a prospective biofuel crop, J. curcas. The aetiology of this disease has not been resolved. Here, we report the complete nucleotide sequences of a Jatropha virus isolated from Dharwad, Southern India. Phylogenetic analysis of the virus genome suggests it is a new strain of Indian cassava mosaic virus. Agroinfiltration of the two cloned viral DNA components produced systemic infection and typical mosaic symptoms in J. curcas, thereby fulfilling Koch’s postulates. The availability of infectious clones will provide a valuable tool to screen J. curcas cultivars for disease resistance and facilitate the generation of virus-resistant J. curcas plants by transgenic technology.
KeywordsMosaic Disease NcoI Site Cassava Mosaic Disease Flock House Virus Nuclear Shuttle Protein
We thank Drs. Srinivasan Ramachandran (Temasek Life Sciences Laboratory), Ramesh S. Bhat (University of Agricultural Sciences, Dharwad, Karnataka, India) and A. S. Byadgi (University of Agricultural Sciences, Dharwad, Karnataka, India) for their kind assistance in collecting J. curcas samples. We also thank the two anonymous reviewers whose helpful suggestions have led to an improvement of our manuscript. This work was supported by the Temasek Life Sciences Laboratory and the Singapore Millennium Foundation.
- 3.Deng F, McGrath PF, Robinson DJ, Harrison BD (1994) Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase reaction with degenerate primers. Ann Appl Biol 125: 327–336Google Scholar
- 11.Raj SK, Snehi SK, Kumar S, Khan MS, Pathre U (2008) First molecular identification of a begomovirus in India that is closely related to Cassava mosaic virus and causes mosaic and stunting of Jatropha curcas L. Australas Plant Dis Notes 3:69–72Google Scholar