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A novel natural mutation in HC-Pro responsible for mild symptomatology of Zucchini yellow mosaic virus (ZYMV, Potyvirus) in cucurbits

Archives of Virology volume 155, pages 397–401 (2010)Cite this article

Abstract

A natural mild isolate of Zucchini yellow mosaic virus was found to contain a mutation in the helper component (HC-Pro) within a conserved motif, “CDNQLD”, located 12 residues downstream from the “FRNK” motif involved in symptom severity. Introducing the mutation in an infectious cDNA clone of ZYMV resulted in an almost complete absence of symptoms, although viral accumulation was only partially reduced. The FRNK(X)12CDNQLD sequence might be part of a larger motif that is conserved in potyviruses and plays a role in symptomatology and/or silencing inhibition.

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Acknowledgments

We thank Dr A. Gal-On for ZYMV cDNA clone and Dr H. Huet for obtaining ZYMV-A-FINK.

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Authors and Affiliations

  1. INRA, Unité de Pathologie Végétale UR407, 84140, Montfavet, France

    Cecile Desbiez, M. Girard & H. Lecoq

Authors
  1. Cecile Desbiez
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  2. M. Girard
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  3. H. Lecoq
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Correspondence to Cecile Desbiez.

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Desbiez, C., Girard, M. & Lecoq, H. A novel natural mutation in HC-Pro responsible for mild symptomatology of Zucchini yellow mosaic virus (ZYMV, Potyvirus) in cucurbits. Arch Virol 155, 397–401 (2010). https://doi.org/10.1007/s00705-009-0569-4

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  • DOI: https://doi.org/10.1007/s00705-009-0569-4

Keywords

  • Melon
  • Zucchini Yellow Mosaic Virus
  • Mild Strain
  • Silence Inhibition
  • Helper Component