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Structure and genome organization of the large RNA of iris yellow spot virus (genus Tospovirus, family Bunyaviridae)

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Abstract

The structure and organization of the large (L) RNA of iris yellow spot virus (IYSV) was determined, and with this report, the complete genomic sequence of IYSV of the genus Tospovirus, family Bunyaviridae has been elucidated. The L RNA of IYSV was 8,880 nucleotides in length and contained a single open reading frame in the viral complementary (vc) strand. The primary translation product of 331.17 kDa shared many of the features of the viral RNA-dependent RNA polymerase (RdRp) coded by L RNAs of known tospoviruses. The 5′ and 3′ termini of IYSV L RNA (vc) contain two untranslated regions of 33 and 226 nucleotides, respectively, and both termini have conserved terminal nucleotides, another common feature of tospovirus genomic RNAs. Conserved motifs characteristic of RdRps of members of the family Bunyaviridae were present in the IYSV RdRp.

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Acknowledgments

This research was supported in part by funding from the USDA-CSREES Western Region IPM Grants Program (#2007-03622) and Specialty Crops Research Initiative. PPNS No. 0505, Department of Plant Pathology, College of Agricultural, Human and Natural Resource Sciences, Agricultural Research Center, Project No. WNP0 0545, Washington State University, Pullman, WA 99164-6430, USA.

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Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, P.O. Box 646430, Pullman, WA, 99164-6430, USA

    Sudeep Bag, K. L. Druffel & H. R. Pappu

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  1. Sudeep Bag
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  2. K. L. Druffel
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  3. H. R. Pappu
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Correspondence to H. R. Pappu.

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The sequence reported here is available in GenBank under accession number FJ623474.

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Bag, S., Druffel, K.L. & Pappu, H.R. Structure and genome organization of the large RNA of iris yellow spot virus (genus Tospovirus, family Bunyaviridae). Arch Virol 155, 275–279 (2010). https://doi.org/10.1007/s00705-009-0568-5

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  • DOI: https://doi.org/10.1007/s00705-009-0568-5

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