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Segment-2 sequence analysis and cross-neutralization studies on some Indian bluetongue viruses suggest isolates are VP2-variants of serotype 23

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Abstract

Sequence analysis of segment 2 (seg-2) of three Indian bluetongue virus (BTV) isolates, Dehradun, Rahuri and Bangalore revealed 99% nucleotide identity amongst them and 96% with the reference BTV 23. Phylogenetic analysis grouped the isolates in ‘nucleotype D’. The deduced amino acid (aa) sequence of the Bangalore isolate showed a high variability in a few places compared to other isolates. B-cell epitope analyses predicted an epitope that is present exclusively in the Bangalore isolate. Two-way cross serum neutralization confirmed that Bangalore isolate is antigenically different from the other two isolates. The results of this study suggest that these three isolates are VP2 variants of BTV 23. This signifies that non-cross-neutralizing variants of the same BTV serotype should be included in vaccine preparation.

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References

  1. Bonneau KR, Zhang N, Zhu J, Zhang F, Li Z, Zhang K, Xiao L, Xiang W, MacLachlan NJ (1999) Sequence comparison of the L2 and S10 genes of bluetongue viruses from the United States and the People’s Republic of China. Virus Res 61:153–160

    Article  CAS  PubMed  Google Scholar 

  2. Clarke IN, McCrae MA (1981) A rapid and sensitive method for analyzing the genome profiles of field isolates of rotavirus. J Virol Methods 2:203–209

    Article  CAS  PubMed  Google Scholar 

  3. Cowley JA, Gorman BM (1989) Cross-neutralization of genetic reassortants of bluetongue virus serotypes 20 and 21. Vet Microbiol 19:37–51

    Article  CAS  PubMed  Google Scholar 

  4. DeMatto CA, DeMattos CCP, Osburn IB, MacLachlan NJ (1994) Heterogeneity of the L2 gene of field isolates of bluetongue virus serotype-17 from the San-Joaquin valley of California. Virus Res 31:67–87

    Article  Google Scholar 

  5. DeMattos CCP, DeMattos CA, Osburn IB, MacLachlan NJ (1994) Evolution of the L2 gene of strains of bluetongue virus serotype-10 isolated in California. Virology 201:173–177

    Article  CAS  Google Scholar 

  6. DeMaula CD, Heinder HW, Rossitto PV, Peirce CM, MacLachlan NJ (1993) Neutralization determinants of United States bluetongue virus serotype 10. Virology 195:292–296

    Article  CAS  PubMed  Google Scholar 

  7. Enserink M (2006) Emerging infectious diseases. During a hot summer, bluetongue virus invades northern Europe. Science 313:1218–1219

    Article  CAS  PubMed  Google Scholar 

  8. Erasmus BJ (1990) Bluetongue virus. In: Dinter Z, Morein B (eds) Virus infection of ruminants, vol 3. Elsevier, Amsterdam, New York, pp 227–237

    Google Scholar 

  9. Felsenstein J (1985) Confidence limits on phylogenesis: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  10. Gould AR, Pritchard LI (1990) Relationships amongst bluetongue viruses revealed by comparisons of capsid and outer coat protein nucleotide sequences. Virus Res 17:31–52

    Article  CAS  PubMed  Google Scholar 

  11. Hamblin C (2004) Bluetongue virus antigen and antibody detection, and the application of laboratory diagnostic techniques. Vet Ital 40:538–545

    Google Scholar 

  12. Jain NC, Sharma R, Prasad G (1986) Isolation of bluetongue virus from sheep in India. Vet Rec 119:17–18

    CAS  PubMed  Google Scholar 

  13. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:783–791

    Article  Google Scholar 

  14. Knudson DL, Butterfield WK, Shope RE, Walton TE, Campbell CH (1982) Electrophoresis comparisons of the genomes of North American bluetongue viruses, one Australian bluetongue virus and three other related orbivirus. Vet Microbiol 7:285–293

    Article  CAS  PubMed  Google Scholar 

  15. Koekemoer JJO, Paweska JT, Pretorus PJ, van Dijk AA (2003) VP2 gene phylogenetic characterization of field isolates of African horse sickness virus serotype 7 circulating in South Africa during the time of the 1999 African horsesickness outbreak in the Western cape. Virus Res 93:159–167

    Article  CAS  PubMed  Google Scholar 

  16. Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. J Gen Virol 88:621–630

    Article  CAS  PubMed  Google Scholar 

  17. McColl KA, Gould AR (1991) Detection and characterization of bluetongue virus using the polymerase chain reaction. Virus Res 21:19–34

    Article  CAS  PubMed  Google Scholar 

  18. Mehrotra ML, Shukla DC, Kataria JM (1989) Isolation and identification of viral agent resembling bluetongue virus in an outbreak in sheep. Indian J Anim Sci 59:1072–1074

    Google Scholar 

  19. Mehrotra ML, Shukla DC, Singh KP, Khanna PN, Saikumar G (1995) Isolation of bluetongue virus from an outbreak of Morbillivirus infection in sheep and goat. J Comp Microbiol Immunol Infect Dis 16:135–136

    Google Scholar 

  20. Mertens PPC, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S (2007) The design of primers and use of RT-PCR assays for typing European BTV isolates: differentiation of field and vaccine strains. J Gen Virol 88:2811–2823

    Article  CAS  PubMed  Google Scholar 

  21. Mertens PPC, Maan S, Samuel A, Attoui H (2004) Oribivirus, Reoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Dseelberger U, Ball LA (eds) Virus taxonomy. VIII Report of the ICTV. Elsevier/Academic Press, London, pp 466–483

    Google Scholar 

  22. Mertens PPC, Pedley S, Cowley J, Burrough JN, Cortey AH, Jeggo MH, Jenning DM, Gorman BM (1989) Analysis of the role of bluetongue virus outer capsid proteins VP2 and VP5 in determination of virus serotype. Virology 170:561–565

    Article  CAS  PubMed  Google Scholar 

  23. Mertens PPC, Sangar DV (1985) Analysis of the terminal sequences of the genome segments of four orbiviruses. Virology 140:55–67

    Article  CAS  PubMed  Google Scholar 

  24. Mondal B, Sen A, Chand K, Biswas SK, De A, Rajak KK, Chakravarti S (2009) Evidence of mixed infection of peste des petits ruminants virus and bluetongue virus in a flock of goats as confirmed by detection of antigen, antibody and nucleic acid of both the viruses. Trop Anim Health Prod 41:1661–1667

    Google Scholar 

  25. Odorico M, Pellequer JL (2003) BEPITOPE: predicting the location of continuous epitope and patterns in proteins. J Mol Recognit 16:20–22

    Article  CAS  PubMed  Google Scholar 

  26. Paul E, Gibbs J, Greiner EC (1994) The epidemiology of bluetongue. Comp Immunol Microbiol Infect Dis 17:207–220

    Article  Google Scholar 

  27. Pierce CM, Rossitto PV, MacLachla NJ (1995) Homotypic and heterotypic neutralization determinants of bluetongue virus serotype 17. Virology 209:63–267

    Article  Google Scholar 

  28. Prasad G, Garg AK, Minakshi KakkerNK, Srivastava RN (1994) Isolation of bluetongue virus from sheep in Rajasthan state. Rev Sci Tech Off Int Epic 13:935–938

    CAS  Google Scholar 

  29. Prichard IL, Daniels PW, Melville LF, Kirkland PD, Johnson SJ, Lunt R, Eaton BT (2004) Genetic diversity of bluetongue viruses in Australia. Vet Ital 40:438–444

    Google Scholar 

  30. Saitou N, Nei M (1987) The neighbor joining method: a new method for reconstructing phylogenetic trees. Mol Biol 4:406–425

    CAS  Google Scholar 

  31. Schwartz-Cornil I, Mertens PPC, Contreras V, Hemati B, Pascale F, Breard E, Mellor PS, MacLachlan NJ, Zientara S (2008) Bluetongue virus: virology, pathogenesis and immunity. Vet Res 39:46. doi:10.1051/vetres:2008023

    Article  PubMed  Google Scholar 

  32. Singh KP, Maan S, Samuel AR, Rao S, Meyer AM, Mertens PPC (2004) Phylogenetic analyses of bluetongue virus genome segment 6 (encoding VP 5) from different serotypes. Vet Ital 40:479–483

    Google Scholar 

  33. Squire KRE, Chuang RY, Osburn BI, Knudson DL, Doi RH (1983) Rapid methods for comparing the double-stranded RNA genome profiles of bluetongue virus. Vet Microbiol 8:543–553

    Article  CAS  PubMed  Google Scholar 

  34. Sreenivasulu D, Subba Rao MV, Reddy YN, Gard GP (2004) Overview of bluetongue disease, viruses, vectors, surveillance and unique features: the Indian sub-continent and adjacent regions. Vet Ital 40:73–77

    Google Scholar 

  35. Sreenivasulu D, Rao MV, Gard GP (1999) Isolation of bluetongue virus serotype 2 from native sheep in India. Vet Rec 144:452–453

    CAS  PubMed  Google Scholar 

  36. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  37. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  Google Scholar 

  38. Toussaint JF, Sailleau C, Mast J, Houdart P, Czaplicki G, Demeestere L, VandenBussche F, van Dessel W, Goris N, Breard E, Bounaadja L, Etienne T, Zientara S, De Clercq K (2007) Bluetongue in Belgium, 2006. Emerg Infect Dis 13:614–616

    Article  PubMed  Google Scholar 

  39. Walton TE (2004) The history of bluetongue and a current global overview. Vet Ital 40:31–38

    Google Scholar 

  40. Wilson WC, Bernard KA, Israel BA, Mecham JO (2008) Bluetongue virus serotype 17 sequence variation associated with neutralization. DNA Seq 19:237–240

    Article  CAS  PubMed  Google Scholar 

  41. Zientara S, Breard E, Sailleau C (2006) Bluetongue: characterization of virus types by reverse transcription-polymerase chain reaction. Dev Biol (Basel) 126:187–196

    CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge Dr. Gaya Prasad for providing the BTV Banglore isolate used in this work. This study was partly supported by the All India Network Program on Bluetongue Disease (ICAR). P. A. Tembhurne and K. B. Pathak were supported by a fellowship from IVRI. The authors also acknowledge J. K. Mohapatra for help with phylogenetic analysis and manuscript correction.

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Authors and Affiliations

  1. National AIDS Research Center, Bhosari, Pune, 411026, Maharashtra, India

    Prabhakar A. Tembhurne

  2. Molecular Virology Lab, 201F Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546-0312, USA

    Kunj B. Pathak

  3. Department of Animal Husbandry and Dairying, Ministry of Agriculture, Government of India, Krishi Bhavan, New Delhi, 110001, India

    Santanu K. Bandyopadhyay

  4. National Research Center for Equines, Sirsa Road, Hisar, Haryana, India

    Raj K. Singh

  5. Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital, 263138, Uttarakhand, India

    Prabhakar A. Tembhurne, Bimalendu Mondal, Kunj B. Pathak, Sanchay K. Biswas, Mahendra P. Yadav, Santanu K. Bandyopadhyay & Raj K. Singh

  6. Central FMD Typing Laboratory, Project Directorate on Foot and Mouth Disease, Mukteswar, Nainital, 263138, Uttarakhand, India

    Aniket Sanyal

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  1. Prabhakar A. Tembhurne
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Correspondence to Bimalendu Mondal.

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Tembhurne, P.A., Mondal, B., Pathak, K.B. et al. Segment-2 sequence analysis and cross-neutralization studies on some Indian bluetongue viruses suggest isolates are VP2-variants of serotype 23. Arch Virol 155, 89–95 (2010). https://doi.org/10.1007/s00705-009-0553-z

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  • DOI: https://doi.org/10.1007/s00705-009-0553-z

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