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Novel monoclonal antibodies against Menangle virus nucleocapsid protein

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Abstract

Menangle virus (MenV) is a member of the family Paramyxoviridae isolated in Australia that causes a reproductive disease of pigs. There is a need for specific immunoassays for virus detection to facilitate the diagnosis of MenV infection. Three novel monoclonal antibodies (MAbs) of the IgG1 subtype were generated by immunizing mice with recombinant yeast-expressed MenV nucleocapsid (N) protein self-assembled to nucleocapsid-like structures. One MAb was cross-reactive with recombinant N protein of Tioman virus. The epitopes of MAbs were mapped using a series of truncated MenV N proteins lacking the 29–119 carboxy-terminal amino acid (aa) residues. The epitopes of two MAbs were mapped to aa 430–460 of the MenV N protein, whilst the epitope of one MAb was mapped to residues 460–490. All three MAbs specifically recognized MenV, as indicated by immunohistochemical staining of brain tissue isolated from a field case (a stillborn piglet) of MenV infection. The MAbs against MenV N protein may be a useful tool for immunohistological diagnosis of MenV infection.

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Acknowledgments

The work was partially supported by the Lithuanian Science and Study Foundation (grants B-36/2008, B-16/2006) and by the Lithuanian Science Council Student Research Fellowship Award. We are grateful to Tony Ross (formerly of Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia) for providing paraffin-wax-embedded sections of still-born piglet tissues from a field case of MenV infection. We also thank Jean Payne and Jenni Rookes (Australian Animal Health Laboratory) for their assistance with immunohistochemical analyses.

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Authors and Affiliations

  1. Institute of Biotechnology, V. Graiciuno 8, 02241, Vilnius, Lithuania

    Aurelija Zvirbliene, Indre Kucinskaite-Kodze, Mindaugas Juozapaitis, Rita Lasickiene, Dalia Gritenaite & Kestutis Sasnauskas

  2. CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, VIC, 3220, Australia

    Gail Russell, John Bingham & Wojtek P. Michalski

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  1. Aurelija Zvirbliene
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  2. Indre Kucinskaite-Kodze
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  3. Mindaugas Juozapaitis
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  4. Rita Lasickiene
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  5. Dalia Gritenaite
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  6. Gail Russell
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  7. John Bingham
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  8. Wojtek P. Michalski
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  9. Kestutis Sasnauskas
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Correspondence to Aurelija Zvirbliene.

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Zvirbliene, A., Kucinskaite-Kodze, I., Juozapaitis, M. et al. Novel monoclonal antibodies against Menangle virus nucleocapsid protein. Arch Virol 155, 13–18 (2010). https://doi.org/10.1007/s00705-009-0543-1

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  • DOI: https://doi.org/10.1007/s00705-009-0543-1

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