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A rapid method for detection of PrP by surface plasmon resonance (SPR)

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Abstract

Surface plasmon resonance was used to develop a rapid, label-free and sensitive immunoassay for detection of Prion protein (PrP). Anti-PrP monoclonal antibodies immobilized on the biosensor surface were allowed to bind various concentrations of cellular prion protein (PrPC), followed by a pulse with additional soluble anti-PrP polyclonal antibodies to intensify the signal. The interaction of antibody with antigen was monitored in real time. With this method, it was possible to detect PrPC with a limit of 31.7 ng/ml in serum and 13.1 ng/ml in HEPES-saline, requiring 1 h for a single sample measurement. The assay also detected misfolded prion protein (PrPSc) in spiked serum and PrPSc in scrapie-infected mouse brains. This is a rapid and sensitive assay for the detection of PrP in serum that could be developed into a platform for a serum-based TSE test.

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Abbreviations

PrP:

Prion protein

PrPC :

Cellular prion protein

PrPSc :

Misfolded prion protein

SPR:

Surface plasmon resonance

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Authors and Affiliations

  1. Institute of Military Veterinary Sciences, Academy of Military Medical Sciences, 1068 Qinglong Road, 130062, Changchun, China

    Wan Jiayu, Wang Xiong, Li Jiping, Liu Wensen, Xu Ming, Liu Linna, Xu Jing, Wang Haiying & Gao Hongwei

Authors
  1. Wan Jiayu
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  2. Wang Xiong
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  3. Li Jiping
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  4. Liu Wensen
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  5. Xu Ming
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  7. Xu Jing
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  8. Wang Haiying
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  9. Gao Hongwei
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Correspondence to Gao Hongwei.

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Jiayu, W., Xiong, W., Jiping, L. et al. A rapid method for detection of PrP by surface plasmon resonance (SPR). Arch Virol 154, 1901–1908 (2009). https://doi.org/10.1007/s00705-009-0532-4

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  • DOI: https://doi.org/10.1007/s00705-009-0532-4

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