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Genetic variability and phylogenetic analysis of hosta virus X

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Abstract

Triple gene block 1 (TGB1) and coat protein (CP) sequences of 30 hosta virus X (HVX) isolates from Tennessee (TN), USA, were determined and compared with available sequences in GenBank. The CPs of all known HVX isolates, including those from TN, shared 98.3–100% and 98.2–100% nucleotide and amino acid sequence identity, respectively, whereas TGB1 shared 97.4–100% nucleotide and 97–100% amino acid sequence identity. TGB1 of TN isolates were all longer by one codon from that of a Korean isolate, which is the only sequence publicly available. Phylogenetic analysis of nucleotide and amino acid sequences of TGB1 and CP of all known HVX isolates, separately or combined, revealed a close relationship, suggesting that all of them are derived from a common ancestor. Phylogenetic analysis with the type member of each genus of the family Flexiviridae confirmed that HVX is a member of a distinct species of the genus Potexvirus.

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Acknowledgments

The authors are grateful to Dr. BEL Lockhart (University of Minnesota, MN, USA) for providing antiserum against HVX and Dr. M. Mazarei (University of Tennessee) for critical reading of the manuscript. This study was supported by USDA-Agriculture Research Service Cooperative Agreement 58-6404-2-0057.

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Authors and Affiliations

  1. Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, 37996, USA

    O. L. Fajolu, R.-H. Wen, A. S. Windham, M. T. Windham, J. K. Moulton & M. R. Hajimorad

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  1. O. L. Fajolu
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  2. R.-H. Wen
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  3. A. S. Windham
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  4. M. T. Windham
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  5. J. K. Moulton
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  6. M. R. Hajimorad
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Corresponding author

Correspondence to M. R. Hajimorad.

Additional information

The nucleotide sequence data of CP and TGB1 genes reported in this article have been deposited in GenBank under accession numbers FJ903386–FJ903415 and FJ903416–FJ903445, respectively.

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Fajolu, O.L., Wen, RH., Windham, A.S. et al. Genetic variability and phylogenetic analysis of hosta virus X. Arch Virol 154, 1909–1916 (2009). https://doi.org/10.1007/s00705-009-0522-6

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  • DOI: https://doi.org/10.1007/s00705-009-0522-6

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