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Identification of two RNA silencing suppressors from banana bunchy top virus

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Abstract

In order to suppress RNA silencing, many plant and some animal viruses encode RNA silencing suppressors to achieve infection. In this study, we report that B3 and B4, encoded by DNA3 and DNA4 of banana bunchy top virus (BBTV), exhibit RNA silencing suppression activity. B3 and B4 were able to increase the transient expression of green fluorescent protein (GFP) and dramatically enhanced the pathogenicity of potato virus X (PVX) in Nicotiana benthamiana. B4 was able to reverse established gene silencing on an inoculated leaf or on an upper leaf. B3, however, was only active during infection of an inoculated leaf. Furthermore, B4, but not B3, was able to enhance GFP expression in the transgenic N. benthamiana line 16c. In conclusion, B3 and B4 are the RNA silencing suppressors of BBTV, and they may act at different steps in the RNA silencing pathways.

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Acknowledgments

We thank Professor Andrew O. Jackson (Department of Plant & Microbial Biology, University of California, Berkeley) for his helpful suggestions and constructive criticisms. This work was supported by grant KL200505 from State Key Laboratory Opening Foundation of China and by grant 80670 from Natural Science Foundation of Hainan.

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Authors and Affiliations

  1. Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, 571101, Haikou, Hainan, China

    Shengniao Niu, Jianhua Wang & Zhixin Liu

  2. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, 100094, Beijing, China

    Baosheng Wang, Xiaofen Guo, Jialin Yu, Xianbing Wang, Kai Xu & Yafeng Zhai

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  1. Shengniao Niu
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Correspondence to Zhixin Liu.

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Niu, S., Wang, B., Guo, X. et al. Identification of two RNA silencing suppressors from banana bunchy top virus. Arch Virol 154, 1775–1783 (2009). https://doi.org/10.1007/s00705-009-0515-5

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  • DOI: https://doi.org/10.1007/s00705-009-0515-5

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