Identification and molecular characterization of a marafivirus in Rubus spp.
- 189 Downloads
An undescribed virus with isometric particles and a diameter of ca. 30 nm was identified in diseased samples of wild and cultivated Rubus species and molecularly characterized. Its genome was 6,463 nt, excluding the 3′-terminal poly(A) tail, and contained a single open reading frame coding for a 2,035-amino-acid-long precursor polypeptide (p223). The amino terminal portion of p223, identified as a replication-associated polyprotein, contained conserved motifs of methyltransferase, endopeptidase/protease, helicase and RNA-dependent RNA polymerase. The carboxy terminus of the large polypeptide is involved in the formation of two viral coat protein subunits with deduced molecular masses of 23 and 21 kDa. Pairwise comparisons and phylogenetic analyses showed closest relationships of this virus with oat blue dwarf virus and citrus sudden death-associated virus, sharing levels of genome sequence conservation far below the species demarcation level established for tymovirids. Our data indicate that this virus, for which the name blackberry virus S (BlVS) is proposed, is a hitherto undescribed species of the genus Marafivirus, family Tymoviridae. A survey conducted in Mississippi, USA, has shown that BlVS is also present in cultivated Rubus germplasm. This work represents the first report of a marafivirus infecting small fruits.
- 3.Banttari EE, Zeyen RJ (1973) Oat blue dwarf virus. CMI/AAB Description Plant Viruses 123Google Scholar
- 5.Dreher TW, Edwards MC, Gibbs AJ, Haenni A-L, Hammond RW, Jupin I, Koenig R, Sabanadzovic S, Abou Ghanem-Sabanadzovic N, Martelli GP (2005) Family Tymoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Eight report of the international committee on taxonomy of viruses. Elsevier, Amsterdam, pp 1061–1074Google Scholar
- 7.Foissac X, Svanella-Dumas L, Gentit P, Dulucq M-J, Marais A, Candresse T (2005) Polyvalent degenerate oligonucleotides reverse transcription-polymerase chain reaction: a polyvalent detection and characterization tool for trichoviruses, capilloviruses, and foveaviruses. Phytopathology 95:617–625CrossRefPubMedGoogle Scholar
- 14.Maccheroni W, Alegria MC, Greggio CC, Piazza JP, Kamla RF, Zacharias PRA, Bar-Joseph M, Kitajima EW, Assumpção LC, Camarotte G, Cardozo J, Casagrande EC, Ferrari F, Franco SF, Giachetto PF, Girasol A, Jordão H Jr, Silva VHA, Souza LCA, Aguilar-Vildoso CI, Zanca AS, Arruda P, Kitajima JP, Reinach FC, Ferro JA, da Silva ACR (2005) Identification and genomic characterization of a new virus (Tymoviridae family) associated with citrus sudden death disease. J Virol 79:3028–3037CrossRefPubMedGoogle Scholar
- 17.Page RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Comp App Biol Sci 12:357–358Google Scholar
- 21.Sabanadzovic S, Abou Ghanem-Sabanadzovic N (2008) Molecular characterization and detection of a tripartite cryptic virus from rose. J Plant Path 90:287–293Google Scholar
- 22.Sabanadzovic S, Abou Ghanem-Sabanadzovic N, Gorbalenya AE (2009) Permutation of the active site of putative RNA-dependent RNA polymerase in a newly identified species of plant Alpha-like virus. Virology. doi:10.1016/j.virol.2009.08.006