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Detection of Aichi virus shedding in a child with enteric and extraintestinal symptoms in Hungary

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Abstract

Aichi virus, genus Kobuvirus, family Picornaviridae, has been proposed as a causative agent of gastroenteritis in human. Although high seroprevalence has been detected, it has been identified in only a few cases. We report detection of Aichi virus in Hungary. A total of 65 stool samples were tested retrospectively, collected from children with diarrhea, by reverse transcription-polymerase chain reaction. One (1.5%) sample from a 3-year-old girl was positive. Besides diarrhea, fever, purulent conjunctivitis and respiratory symptoms were also present at the same time with virus shedding. The genotype A virus, Kobuvirus/human/Szigetvar-HUN298/2000/Hungary (FJ225407), has 96% nucleotide identity to Aichi virus.

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References

  1. Ambert-Balay K, Lorrot M, Bon F, Giraudon H, Kaplon J, Wolfer M, Lebon P, Gendrel D, Pothier P (2008) Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients. J Clin Microbiol 46:1252–1258

    Article  PubMed  CAS  Google Scholar 

  2. Goyer M, Aho L-S, Bour J-B, Ambert-Balay K, Pothier P (2008) Seroprevalence distribution of Aichi virus among a French population in 2006–2007. Arch Virol 153:1171–1174

    Article  PubMed  CAS  Google Scholar 

  3. Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17:1244–1245

    Article  PubMed  CAS  Google Scholar 

  4. Nicholas KB, Nicholas HB Jr (1997) GeneDoc: a tool for editing and annotating multiple sequence alignments

  5. Oh DY, Silva PA, Hauroeder B, Deidrich S, Cardoso DD, Schreier E (2006) Molecular characterization of the first Aichi viruses isolated in Europe and in South America. Arch Virol 151:1199–1206

    Article  PubMed  CAS  Google Scholar 

  6. Pham NT, Khamrin P, Nguyen TA, Kanti DS, Phan TG, Okitsu S, Ushijima H (2007) Isolation and molecular characterization of Aichi viruses from fecal specimens collected in Japan, Bangladesh, Thailand, and Vietnam. J Clin Microbiol 45:2287–2288

    Article  PubMed  CAS  Google Scholar 

  7. Reuter G, Boldizsár Á, Kiss I, Pankovics P (2008) Candidate new species of Kobuvirus in porcine hosts. Emerg Infect Dis 12:1968–1970

    Article  Google Scholar 

  8. Reuter G, Boldizsár Á, Pankovics P (2009) Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in genus Kobuvirus, family Picornaviridae. Arch Virol 154:101–108

    Article  PubMed  CAS  Google Scholar 

  9. Reuter G, Egyed L (2009) Bovine kobuviruses in Europe. Emerg Infect Dis 5:822–823

    Article  Google Scholar 

  10. Reuter G, Farkas T, Berke T, Jiang X, Matson DO, Szűcs Gy (2002) Sapporo-szerű vírusok ismeretlen kóreredetű szórványos gastroenteritisekben (Sapporo-like viruses in sporadic gastroenteritis with unknown origin). Orv Hetil 7:351–354

    Google Scholar 

  11. Sdiri-Loulizi K, Gharbi-Khélifi H, de Rougemont A, Chouchane S, Sakly N, Ambert-Balay K, Hassine M, Guédiche MN, Aouni M, Pothier P (2008) Acute infantile gastroenteritis associated with human enteric viruses in Tunisia. J Clin Microbiol 46:1349–1355

    Article  PubMed  Google Scholar 

  12. Yamashita T, Kobayashi S, Sakae K, Nakata S, Chiba S, Ishihara Y, Isomura S (1991) Isolation of cytopathic small round viruses with BS-C-1 cells from patients with gastroenteritis. J Infect Dis 164:954–957

    PubMed  CAS  Google Scholar 

  13. Yamashita T, Ito M, Kabashima Y, Tsuzuki H, Fujiura A, Sakae K (2003) Isolation and characterization of a new species of kobuvirus associated with cattle. J Gen Virol 84:3069–3077

    Article  PubMed  CAS  Google Scholar 

  14. Yamashita T, Sakae K, Ishihara Y, Isomura S, Utagawa E (1993) Prevalence of newly isolated, cytopathic small round virus (Aichi strain) in Japan. J Clin Microbiol 31:2938–2943

    PubMed  CAS  Google Scholar 

  15. Yamashita T, Sakae K, Tsuzuki H, Suzuki Y, Ishikawa N, Takeda N, Miyamura T, Yamazaki S (1998) Complete nucleotide sequence and genetic organization of Aichi virus, a distinct member of the Picornaviridae associated with acute gastroenteritis in humans. J Virol 72:8408–8412

    PubMed  CAS  Google Scholar 

  16. Yamashita T, Sugiyama M, Tsuzuki H, Sakae K, Suzuki Y, Miyazaki Y (2000) Application of a reverse transcription-PCR for identification and differentiation of Aichi virus, a new member of the Picornavirus family associated with gastroenteritis in humans. J Clin Microbiol 38:2955–2961

    PubMed  CAS  Google Scholar 

  17. Yamashita T, Sakae K, Kobayashi S, Ishihara Y, Miyake T, Mubina A, Isomura S (1995) Isolation of cytopathic small round virus (Aichi virus) from Pakistani children and Japanese travelers from Southeast Asia. Microbiol Immunol 39:433–435

    PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Teruo Yamashita for kindly providing an Aichi virus prototype strain A846/88 in year 2001. This work was supported by grants from the Hungarian Scientific Research Fund (OTKA, F048433), and the project “Enteric Virus Emergence, New Tools” (EVENT, SP22-CT-2004-502571) funded by the European Union.

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Authors and Affiliations

  1. Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Szabadság út 7, 7623, Pecs, Hungary

    Gábor Reuter, Ákos Boldizsár & Péter Pankovics

  2. Szigetvár, Hungary

    Gábor Papp

Authors
  1. Gábor Reuter
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  2. Ákos Boldizsár
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  3. Gábor Papp
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  4. Péter Pankovics
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Corresponding author

Correspondence to Gábor Reuter.

Additional information

Nucleotide sequence data reported are available in the GenBank database under accession number FJ225407.

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Reuter, G., Boldizsár, Á., Papp, G. et al. Detection of Aichi virus shedding in a child with enteric and extraintestinal symptoms in Hungary. Arch Virol 154, 1529–1532 (2009). https://doi.org/10.1007/s00705-009-0473-y

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  • DOI: https://doi.org/10.1007/s00705-009-0473-y

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