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Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus Panicovirus

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Abstract

The complete nucleotide sequence of the Phleum isolate of cocksfoot mild mosaic virus (CMMV-P) and the coat protein sequence of the cocksfoot isolate (CMMV-1) were determined. Comparative sequence analysis revealed a close relationship with Panicum mosaic virus (PMV; genus Panicovirus), and together with serological studies, the work supports the classification of CMMV in the family Tombusviridae, genus Panicovirus rather than, as is currently proposed, the genus Sobemovirus. A full-length cDNA clone was prepared, and RNA transcripts synthesised from cDNA were shown to be infectious when inoculated to Hordeum vulgare.

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Acknowledgments

We thank Professor Karen-Beth Scholthof for providing samples of Panicum mosaic virus and antiserum, Dr. W. Huth for providing samples of CMMV-1, and Karen Jackson for technical assistance. SCRI receives funding from the Scottish Government Research, Analysis and Rural Affairs Department.

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Authors and Affiliations

  1. Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK

    Angelika Ziegler, Graham Cowan & Lesley Torrance

Authors
  1. Angelika Ziegler
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  2. Graham Cowan
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  3. Lesley Torrance
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Corresponding author

Correspondence to Angelika Ziegler.

Additional information

The GenBank accession number of the sequence reported in this paper is EU081018.

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Ziegler, A., Cowan, G. & Torrance, L. Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus Panicovirus . Arch Virol 154, 1545–1549 (2009). https://doi.org/10.1007/s00705-009-0468-8

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  • DOI: https://doi.org/10.1007/s00705-009-0468-8

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