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Characterization of a spontaneous drug-resistant mutant of monkey B virus (Macacine herpesvirus 1)

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Abstract

Monkey B virus (Macacine herpesvirus 1; BV) is an α-herpesvirus of macaques that causes serious infections in humans. A spontaneous mutant resistant to penciclovir (PCV) was isolated. Several genes were sequenced to identify mutations potentially responsible for PCV resistance. A single nucleotide deletion in the thymidine kinase (TK) gene was identified. To confirm its role in PCV resistance, several TK recombinants were constructed. A TK-deletion virus and a recombinant carrying the mutation were both resistant to PCV, while a revertant was PCV-sensitive. These results demonstrate that spontaneous drug-resistant mutants of BV do occur and that the BV TK is responsible for sensitivity to PCV.

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Acknowledgments

This work was supported in part by PHS grant P40 12317.

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Authors and Affiliations

  1. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    Darla H. Black & R. Eberle

  2. Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    Lara K. Maxwell

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  1. Darla H. Black
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  2. Lara K. Maxwell
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  3. R. Eberle
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Correspondence to R. Eberle.

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Black, D.H., Maxwell, L.K. & Eberle, R. Characterization of a spontaneous drug-resistant mutant of monkey B virus (Macacine herpesvirus 1). Arch Virol 154, 1495–1497 (2009). https://doi.org/10.1007/s00705-009-0452-3

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  • DOI: https://doi.org/10.1007/s00705-009-0452-3

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