Virus isolate G10 was obtained from diseased allamanda plants showing leaf curl symptoms in Guangdong, China. The full-length nucleotide sequence of a DNA-A-like molecule of G10 was cloned and sequenced; it comprises 2755 nucleotides and has a typical begomovirus genome organization with six conserved open reading frames. When compared with the DNA-A sequences of other begomoviruses, the complete nucleotide sequence of DNA-A of G10 had the highest sequence identity (81.2%) to tomato leaf curl Guangdong virus (ToLCGuV) isolate G2. This is less than the 89% identity in the complete genome that has been defined as the threshold value for demarcation of species in the genus Begomovirus. The molecular data show that isolate G10 from allamanda in Guangdong, China is a distinct Begomovirus species, for which the name Allamanda leaf curl virus (AlLCV) is proposed.
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Briddon RW, Markham PG (2000) Cotton leaf curl virus disease. Virus Res 71:151–159
Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG (2001) Identification of DNA components required for induction of cotton leaf curl disease. Virology 285:234–243
Cui XF, Tao XR, Xie Y, Fauquet CM, Zhou XP (2004) A DNAβ associated with tomato yellow leaf curl China virus is required for symptom induction. J Virol 78:13966–13974
Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou XP (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821
He ZF, Yu H, Luo FF (2004) Detection of whitefly-transmitted geminiviruses from tomato by PCR. Virol Sin 19:67–69
He ZF, Mao MJ, Yu H, Wang XM, Li HP (2008) First report of a strain of Alternanthera yellow vein virus infecting Eclipta prostrate (L.) L. (Compositae) in China. J Phytopathol 156:496–498
Hong YG, Cai JH, Wang XF, Wang WH, Huang Y, Tian B (1994) Preliminary study on molecular evolution of tobacco leaf curl geminivirus. Chin Sci Bull 39:520–523
Hong YG, Wang XF, Tian B, Cai JH (1995) Chinese squash leaf curl virus—a new whitefly-transmitted geminivirus. Sci China (Ser B) 38:179–186
Jose J, Usha R (2003) Bhendi yellow vein mosaic disease in India is caused by association of a DNA β satellite with a begomovirus. Virology 305:310–317
Laufs J, Traut W, Heyraud F, Matzeit V, Rogers SG, Schell J, Gronenborn B (1995) In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus. Proc Natl Acad Sci USA 92:3879–3883
Lazarowitz S (1992) Geminiviruses: genome structure and gene function. Crit Rev Plant Sci 11:327–349
Liu YL, Cai JH, Li DL, Qin BX, Tian P (1998) Chinese tomato yellow leaf curl virus–a new species of geminivirus. Sci China C Life Sci 28:148–153
Ma XY, Cai JH, Li GX, Qin BX, Zhou XP (2004) Molecular characterization of a distinct begomovirus infecting Euphorbia pulcherrima in China. J Phytopathol 152:215–218
Rogers SG, Bisaro DM, Horsch RB, Fraley RT, Hoffman NL, Brand L, Elmer JS, Lloyd AM (1986) Tomato golden mosaic virus A component DNA replicates autonomously in transgenic plants. Cell 45:593–600
Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly transmitted geminiviruses. Plant Dis 77:340–347
Saunders K, Bedford ID, Briddon RW, Markham PG, Wong SM, Stanley J (2000) A unique virus complex causes ageratum yellow vein disease. Proc Natl Acad Sci USA 97:6890–6895
Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC (2005) Geminiviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. VIIIth Report Int. Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, pp 301–326
Xie Y, Zhou XP, Zhang ZK, Qi YJ (2002) Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus. Chin Sci Bull 47:197–200
Xiong Q, Fan SW, Wu JX, Zhou XP (2007) Ageratum yellow vein China virus is a distinct begomovirus species associated with a DNA beta molecule. Phytopathology 97:405–411
Wyatt SD, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86:1288–1293
Zhou XP, Xie Y, Tao XR, Zhang ZK, Li ZH, Fauquet CM (2003) Characterization of DNAβ associated with begomoviruses in China and evidence for coevolution with their cognate viral DNA-A. J Gen Virol 84:237–247
This research work was supported by the National Natural Science Foundation of China (Grant No. 30670085). The work was also funded by Natural Science Foundation of Guangdong Province (Grant No. 05103387, 2007A050100005).
The GenBank accession number of the sequence reported in this paper is EF602306.
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He, Z.F., Mao, M.J., Yu, H. et al. Molecular characterization of a distinct begomovirus infecting Allamanda cathartica in Guangdong, China. Arch Virol 154, 1199–1202 (2009). https://doi.org/10.1007/s00705-009-0445-2
- Yellow Vein
- Monopartite Begomoviruses
- Yellow Vein Mosaic Virus
- High Amino Acid Sequence Similarity
- Ageratum Conyzoides