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Genomic sequence of a Japanese encephalitis virus isolate from southern China

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Abstract

We determined the complete nucleotide sequence of a Japanese encephalitis virus (JEV) isolate (designated SH17M-2007) from a pool of Culex tritaeniorhynchus collected in southern China in 2007. The genome consisted of 10,965 nucleotides and included a single open reading frame (10,296 nucleotides) that encodes a 3,432-amino-acid polyprotein. The SH17M-2007 had 97.3 to 98.4% nucleotide identity with two Korean strains (KV1899, K94P05) and two Japanese strains (Ishikawa, JEV/sw/Mie/40/2004), but only 88.8% identity with the Chinese vaccine strain SA14-14-2. Five unique amino acid substitutions including one in the envelope (E) protein (GluE-306-Lys) were found in the SH17M-2007 strain. Phylogenetic relationships based on the full-length nucleotide sequences were similar to those based on the E gene.

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Abbreviations

JEV:

Japanese encephalitis virus

ORF:

Open reading frame

NTR:

Non-translated region

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Acknowledgments

We thank Dr. Yanting Li, Yi He and Yiyi Zhu for assistance in mosquito collection and Dr. Yigang Tong for data analysis. This research was supported by National High-Tech R & D Program (863 Project) (No. 2007AA02Z481) of China, the National Science Fund for Distinguished Young Scholars (No. 30725032) and the National Natural Science Fund of China (No. 30872152).

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Authors and Affiliations

  1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong Da-Street, Fengtai District, 100071, Beijing, People’s Republic of China

    Jiu-Song Zhang, Qiu-Min Zhao, Pan-He Zhang, Na Jia & Wu-Chun Cao

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  1. Jiu-Song Zhang
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  2. Qiu-Min Zhao
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  3. Pan-He Zhang
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  5. Wu-Chun Cao
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Correspondence to Wu-Chun Cao.

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Zhang, JS., Zhao, QM., Zhang, PH. et al. Genomic sequence of a Japanese encephalitis virus isolate from southern China. Arch Virol 154, 1177–1180 (2009). https://doi.org/10.1007/s00705-009-0421-x

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  • DOI: https://doi.org/10.1007/s00705-009-0421-x

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