Abstract
Friend leukemia virus complex consists of a replication-competent virus plus one of two replication-incompetent viruses, spleen focus-forming virus anemia virus or spleen focus-forming virus polycythemia virus. The replication-incompetent viruses induce rapid malignant transformation of erythroid precursor cells. Transformed cell lines from mice infected with the complex can be induced to undergo erythrodifferentiation in vitro. However, lines containing the anemia-type virus require erythropoietin and another agent such as dimethyl sulfoxide for optimal erythrodifferentiation, whereas those containing the polycythemia-type virus do not require or respond to erythropoietin. Mice infected with the original Friend virus isolates were anemic, so sub-lines derived from these mice should be erythropoietin-dependent for induction of erythrodifferentiation. However, many of the widely studied sub-lines are erythropoietin-independent. In order to clarify this apparent anomaly, the genomes of viruses present in two commonly used erythropoietin-independent sub-lines were sequenced. Sequence analysis demonstrates that they contain the polycythemia-type virus and not the anemia-type virus.
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Balta G, Brickner HE, Takegawa S, Kazazian HH Jr, Papayannopoulou T, Forget BG, Atweh GF (1994) Increased expression of the G gamma and A gamma globin genes associated with a mutation in the A gamma enhancer. Blood 83:3727–3737
Constantinescu SN, Keren T, Russ WP, Ubarretxena-Belandia I, Malka Y, Kubatzky KF, Engelman DM, Lodish HF, Henis YI (2003) The erythropoietin receptor transmembrane domain mediates complex formation with viral anemic and polycythemic gp55 proteins. J Biol Chem 278:43755–43763
Deisseroth A, Hendrick D (1978) Human alpha-globin gene expression following chromosomal dependent gene transfer into mouse erythroleukemia cells. Cell 15:55–63
Fang C, Choi E, Nie L, Li JP (1998) Role of the transmembrane sequence of spleen focus-forming virus gp55 in erythroleukemogenesis. Virology 252:46–53
Friend C (1957) Cell-free transmission in adult Swiss mice of a disease having the character of a leukemia. J Exp Med 105:307–318
Friend C (1960) Immunological studies on the leukemia agent of Swiss mice. Acta Unio Int Contra Cancrum 16:1171–1174
Friend C, Preisler HD, Scher W (1974) Studies on the control of differentiation of murine virus-induced erythroleukemic cells. Curr Top Dev Biol 8:81–101
Friend C (1981) Murine virus-induced erythroleukemia cell lines. In: Sato G (ed) Functionally differentiated cell lines. Alan R Liss, New York, pp 235–249
Huang LJ, Constantinescu SN, Lodish HF (2001) The N-terminal domain of Janus kinase 2 is required for Golgi processing and cell surface expression of erythropoietin receptor. Mol Cell 8:1327–1338
Lu M, Xia L, Hua H, Jing Y (2008) Acetyl-keto-beta-boswellic acid induces apoptosis through a death receptor 5-mediated pathway in prostate cancer cells. Cancer Res 68:1180–1186
Mager D, MacDonald ME, Robson IB, Mak TW, Bernstein A (1981) Clonal analysis of the late stages of erythroleukemia induced by two distinct strains of Friend leukemia virus. Mol Cell Biol 1:721–730
Marks PA, Michaeli J, Jackson J, Richon VM, Rifkind RA (1986) Induced differentiation of murine erythroleukemia cells (MELC) by polar compounds: marked increased sensitivity of vincristine resistant MELC. Prog Clin Biol Res 316B:171–181
McClinton D, Stafford J, Brents L, Bender TP, Kuehl WM (1990) Differentiation of mouse erythroleukemia cells is blocked by late up-regulation of a c-myb transgene. Mol Cell Biol 10:705–710
Mirand EA (1967) Erythropoietin-like effect of a polycythemic virus. Proc Soc Exp Biol Med 125:562–565
Ney PA, D’Andrea AD (2000) Friend erythroleukemia revisited. Blood 96:3675–3680
Ohta Y, Tanaka M, Terada M, Miller OJ, Bank A, Marks P, Rifkind RA (1976) Erythroid cell differentiation: murine erythroleukemia cell variant with unique pattern of induction by polar compounds. Proc Natl Acad Sci USA 73:1232–1236
Pogo BG, Friend C (1982) Persistent infection of Friend erythroleukemia cells with vaccinia virus. Proc Natl Acad Sci USA 79:4805–4809
Ramsay RG, Ikeda K, Rifkind RA, Marks PA (1986) Changes in gene expression associated with induced differentiation of erythroleukemia: protooncogenes, globin genes, and cell division. Proc Natl Acad Sci USA 83:6849–6853
Ruscetti SK (1999) Deregulation of erythropoiesis by the Friend spleen focus-forming virus. Int J Biochem Cell Biol 31:1089–1109
Scher BM, Wei XJ, Waxman S, Scher W (1998) Dephosphorylation of Vav is associated with the induction of mouse erythroleukemia cell differentiation: effects of orthovanadate and levamisole. Int J Oncol 12:1307–1313
Takegawa S, Brice M, Stamatoyannopoulos G, Papayannopoulou T (1986) Only adult hemoglobin is produced in fetal nonerythroid x MEL cell hybrids. Blood 68:1384–1388
Waxman S, Scher W, Scher BM (1986) Basic principles for utilizing combination differentiation agents. Cancer Detect Prev 9:395–407
Wolff L, Kaminchik J, Hankins WD, Ruscetti SK (1985) Sequence comparisons of the anemia- and polycythemia-inducing strains of Friend spleen focus-forming virus. J Virol 53:570–578
Acknowledgments
We thank Donald S. Kohtz for advice and George Atweh and Victoria Richon for cell lines. This work was supported by The Samuel Waxman Cancer Research Foundation and The Chemotherapy Foundation.
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The sequences in this paper have been deposited in GenBank with the accession numbers FJ556972 and FJ556973.
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Scher, W., Jing, Y., Lu, M. et al. Sequences of polycythemia-type Friend spleen focus-forming virus in clone-745-derived mouse erythroleukemia cells. Arch Virol 154, 895–898 (2009). https://doi.org/10.1007/s00705-009-0372-2
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DOI: https://doi.org/10.1007/s00705-009-0372-2