Abstract
The amino acid sequence diversity of readthrough proteins (RTPs) of 24 dwarfing isolates of Soybean dwarf virus (SbDV) from Wisconsin and Illinois was analyzed. The RTP, a minor component of viral capsids, has a significant role in specificity of aphid transmission of luteovirids. Among the isolates, nucleotide sequence identities ranged from 95 to 100%. The predicted amino acid sequences differed at 56 amino acid positions in the 54 kDa RTD compared to only five positions in the 22 kDa CP. Phylogenetic analysis of both amino acid and nucleotide sequences showed three distinct clusters of SbDV isolates.
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Acknowledgments
We thank A. Phibbs, and A. Barta for providing SbDV-infected Wisconsin soybean samples. This study was supported by funding from United States Department of Agriculture-Agricultural Research Service and the North Central Soybean Research Program.
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The GenBank accession numbers of the sequence reported in this paper are EU095846, EU095847, and EU419570–EU419584.
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Thekke Veetil, T., Hobbs, H.A. & Domier, L.L. Sequence diversity of readthrough proteins of Soybean dwarf virus isolates from the Midwestern United States. Arch Virol 154, 861–866 (2009). https://doi.org/10.1007/s00705-009-0370-4
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DOI: https://doi.org/10.1007/s00705-009-0370-4