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A longitudinal cohort study in calves evaluated for rotavirus infections from 1 to 12 months of age by sequential serological assays

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Abstract

Using an immunocytochemical staining assay involving six different recombinant baculoviruses with each expressing one of the major bovine rotavirus VP7 (G6, G8 and G10) and VP4 (P6[1], P7[5] and P8[11]) serotypes, we analyzed IgG antibody responses to individual proteins in archival serum samples collected from 31 calves monthly from 1 to 12 months of age during 1974–1975 in Higley, Arizona. Seroresponses to VP7 and VP4, as determined by a fourfold or greater antibody response, were not always elicited concurrently following infection: in some calves, (1) seroresponses to VP7 were detected earlier than to VP4 or vice versa; and (2) a subsequent second seroresponse was detected for VP7 or VP4 only. In addition, a second infection was more likely to be caused by different G and/or P types. Analyses of serum samples showed that the most frequent G–P combination was G8P6[1], followed by G8P7[5], G8P8[11] and G6P6[1].

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Acknowledgments

This work was supported in part by the Intramural Research Program of the National Institute of Allergy and Infectious Disease, National Institutes of Health, USA. There is no conflict of interest to declare.

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Author notes

  1. Blessing Igboeli

    Present address: Case Western Reserve University School of Medicine, Cleveland, OH, USA

  2. Jess L. Ayers

    Present address: Ayers Animal Clinic, Santa Maria, CA, USA

Authors and Affiliations

  1. Epidemiology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 50, Room 6308, Bethesda, MD, 20892, USA

    Dianjun Cao, Blessing Igboeli, Albert Z. Kapikian & Yasutaka Hoshino

  2. Virology and Immunology Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA, 24061, USA

    Lijuan Yuan

  3. Department of Veterinary Science, University of Arizona, Tucson, AZ, 85721, USA

    Jess L. Ayers & Francis R. Abinanti

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  1. Dianjun Cao
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  2. Blessing Igboeli
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  4. Albert Z. Kapikian
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Correspondence to Yasutaka Hoshino.

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Francis R. Abinanti: Deceased.

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Cao, D., Igboeli, B., Yuan, L. et al. A longitudinal cohort study in calves evaluated for rotavirus infections from 1 to 12 months of age by sequential serological assays. Arch Virol 154, 755–763 (2009). https://doi.org/10.1007/s00705-009-0331-y

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