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Iranian beet necrotic yellow vein virus (BNYVV): pronounced diversity of the p25 coding region in A-type BNYVV and identification of P-type BNYVV lacking a fifth RNA species

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Abstract

Beet necrotic yellow vein virus (BNYVV) was detected in 288 of the 392 samples collected in Iran. A-type BNYVV was detected most frequently. The p25 coding region on BNYVV RNA-3 was amplified by RT-PCR and sequenced. Nine different variants of the highly variable amino acid tetrad at positions 67–70 of p25 were identified, i.e. ACHG, AHHG, AYHG, ALHG, AFHR, AFHG, AHYG, VLHG and VHHG. These are more different tetrad variants than have been reported from any other country. The first three variants were found most commonly. In 23 out of the 288 BNYVV-positive samples, we detected P-type BNYVV that had previously been identified only in France, Kazakhstan and recently in the UK. Surprisingly, none of these samples contained the fifth RNA species usually associated with P-type BNYVV in other countries. As in other BNYVV P-type sources, the p25 amino acid tetrad in positions 67–70 of the Iranian P-type consists of SYHG.

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Acknowledgments

This work was supported in part by a scholarship to M. Mehrvar from the Ministry of Science, Research and Technology, Iran, by the Walloon region (DGA, subvention D31-1146) and in part by the Arbeitsgemeinschaft industrieller Forschungsvereinigungen (AiF Grant 14163 N72, GFP BR42/04) in Germany.

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Authors and Affiliations

  1. Université Catholique de Louvain, Unité de Phytopathologie, Louvain-la-Neuve, Belgium

    M. Mehrvar & C. G. Bragard

  2. Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    M. Mehrvar

  3. Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran

    J. Valizadeh

  4. c/o Julius-Kühn-Institut (JKI), Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Braunschweig, Germany

    R. Koenig

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  1. M. Mehrvar
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  2. J. Valizadeh
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  3. R. Koenig
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  4. C. G. Bragard
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Correspondence to C. G. Bragard.

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Mehrvar, M., Valizadeh, J., Koenig, R. et al. Iranian beet necrotic yellow vein virus (BNYVV): pronounced diversity of the p25 coding region in A-type BNYVV and identification of P-type BNYVV lacking a fifth RNA species. Arch Virol 154, 501–506 (2009). https://doi.org/10.1007/s00705-009-0322-z

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  • DOI: https://doi.org/10.1007/s00705-009-0322-z

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