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Infectivity and propagation of attenuated infectious bursal disease virus in the chicken B-lymphocyte cell line DT40

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Abstract

In this paper, the infectivity and propagation of two attenuated infectious bursal disease virus (IBDV) strains in DT40 cells were investigated. The results showed that both of the tested strains, TAD and HN3, directly infect and proliferate in DT40 cells, requiring no adaptive passages. Unexpectedly, IBDV can be rapidly propagated and continuously harvested at high titers for a long time, accompanied by the rapid growth of host cells and showing no increase in pathogenicity. Our results provide further support to suggest that DT40 cells can be used as an ideal model for studying IBDV pathogenesis. Additionally, the DT40 cell line could also serve as a potential system for commercial IBDV vaccine preparation.

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References

  1. Baba TW, Giroir BP, Humphries EH (1985) Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144:139–151

    Article  PubMed  CAS  Google Scholar 

  2. Burkhardt E, Muller H (1987) Susceptibility of chicken blood lymphoblasts and monocytes to infectious bursal disease virus (IBDV). Arch Virol 94:297–303

    Article  PubMed  CAS  Google Scholar 

  3. Chen HY, Zhou Q, Zhang MF et al (1998) Sequence analysis of the VP2 hypervariable region of nine infectious bursal disease virus isolates from mainland China. Avian Dis 42:762–769

    Article  PubMed  CAS  Google Scholar 

  4. Hirai K, Calnek BW (1979) In vitro replication of infectious bursal disease virus in established lymphoid cell lines and chicken B lymphocytes. Infect Immun 25:964–970

    PubMed  CAS  Google Scholar 

  5. Hirai K, Funakoshi T, Nakai T et al (1981) Sequential changes in the number of surface immunoglobulin-bearing B lymphocytes in infectious bursal disease virus-infected chickens. Avian Dis 25:484–496

    Article  PubMed  CAS  Google Scholar 

  6. Hudson L, Pattison M, Thantrey N (1975) Specific B lymphocyte suppression by infectious bursal agent (Gumboro disease virus) in chickens. Eur J Immunol 5:675–679

    Article  PubMed  CAS  Google Scholar 

  7. Kaufer I, Weiss E (1980) Significance of bursa of fabricius as target organ in infectious bursal disease of chickens. Infect Immun 27:364–367

    PubMed  CAS  Google Scholar 

  8. Kibenge FS, McKenna PK (1992) Isolation and propagation of infectious bursal disease virus using the ovine kidney continuous cell line. Avian Dis 36:256–261

    Article  PubMed  CAS  Google Scholar 

  9. Lasher HN, Davis VS (1997) History of infectious bursal disease in the USA—the first two decades. Avian Dis 41:11–19

    Article  PubMed  CAS  Google Scholar 

  10. Lin TW, Lo CW, Lai SY et al (2007) Chicken heat shock protein 90 is a component of the putative cellular receptor complex of infectious bursal disease virus. J Virol 81:8730–8741

    Article  PubMed  CAS  Google Scholar 

  11. McCormack WT, Tjoelker LW, Thompson CB (1991) Avian B-cell development: generation of an immunoglobulin repertoire by gene conversion. Annu Rev Immunol 9:219–241

    Article  PubMed  CAS  Google Scholar 

  12. Müller H, Islam MR, Raue R (2003) Research on infectious bursal disease–the past, the present and the future. Vet Microbiol 97:153–165

    Article  PubMed  Google Scholar 

  13. Ogawa M, Yamaguchi T, Setiyono A et al (1998) Some characteristics of a cellular receptor for virulent infectious bursal disease virus by using flow cytometry. Arch Virol 143:2327–2341

    Article  PubMed  CAS  Google Scholar 

  14. Peters MA, Lin TL, Wu CC (2004) Infectious bursal disease virus polyprotein expression arrests growth and mitogenic stimulation of B lymphocytes. Arch Virol 149:2413–2426

    Article  PubMed  CAS  Google Scholar 

  15. Reed LJ, Muench H (1938) A simple method for estimating fifty percent endpoints. Am J Hyg 27:493–497

    Google Scholar 

  16. Rodriguez-Lecompte JC, Nino-Fong R, Lopez A et al (2005) Infectious bursal disease virus (IBDV) induces apoptosis in chicken B cells. Comp Immunol Microbiol Infect Dis 28:321–337

    Article  PubMed  Google Scholar 

  17. Terasaki K, Hirayama H, Kasanga CJ et al (2008) Chicken B lymphoma DT40 cells as a useful tool for in vitro analysis of pathogenic infectious bursal disease virus. J Vet Med Sci 70:407–410

    Article  PubMed  CAS  Google Scholar 

  18. Tham KM, Moon CD (1996) Apoptosis in cell cultures induced by infectious bursal disease virus following in vitro infection. Avian Dis 40:109–113

    Article  PubMed  CAS  Google Scholar 

  19. Thompson CB, Humphries EH, Carlson LM et al (1987) The effect of alterations in myc gene expression on B cell development in the bursa of Fabricius. Cell 51:371–381

    Article  PubMed  CAS  Google Scholar 

  20. Winding P, Berchtold MW (2001) The chicken B cell line DT40: a novel tool for gene disruption experiments. J Immunol Methods 249:1–16

    Article  PubMed  CAS  Google Scholar 

  21. Zheng XJ, Hong LL, Shi LX et al (2008) Proteomics analysis of host cells infected with infectious bursal disease virus. Mol Cell Proteomics 7:612–625

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Basic Research Program (2005CB523200), National Key Technology R&D Program (2006BAD06A04-6) and Natural Science Foundation of Henan Province (082300433201). The authors also gratefully acknowledge the critical review of the manuscript by Dr. Avraham Rasooly (Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, USA).

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Authors and Affiliations

  1. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, People’s Republic of China

    Jun Luo, Gai-Ping Zhang, Jian-Ming Fan, Man Teng, Lei-Ming You, Ling Zhou, Rui-Guang Deng, Xuan-Nian Wang, Yan-Yan Yang, Li Wang, Guang-Xu Xing & Na Cheng

  2. The Laboratory of Toxicology, College of Public Health, Zhengzhou University, 450001, Zhengzhou, People’s Republic of China

    Jian-Ming Fan

  3. Xinxiang University, 453003, Xinxiang, People’s Republic of China

    Xuan-Nian Wang

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  1. Jun Luo
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  2. Gai-Ping Zhang
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  4. Man Teng
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  6. Ling Zhou
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  7. Rui-Guang Deng
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  8. Xuan-Nian Wang
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  9. Yan-Yan Yang
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  10. Li Wang
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  11. Guang-Xu Xing
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  12. Na Cheng
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Correspondence to Gai-Ping Zhang.

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Luo, J., Zhang, GP., Fan, JM. et al. Infectivity and propagation of attenuated infectious bursal disease virus in the chicken B-lymphocyte cell line DT40. Arch Virol 154, 513–517 (2009). https://doi.org/10.1007/s00705-009-0321-0

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  • DOI: https://doi.org/10.1007/s00705-009-0321-0

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