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The complete nucleotide sequence of potato virus T

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Abstract

Potato virus T (PVT), a member of an unassigned species in the family Flexiviridae, has a genome 6,539 nt in size with three ORFs coding for replication-associated proteins (185 kDa, ORF 1), movement protein (40 kDa, ORF 2) and coat protein (24 kDa, ORF 3), respectively. PVT differs from the type members of all genera of the family Flexiviridae with a 30K-type movement protein and is phylogenetically distant from all of these viruses, least so from grapevine virus A (GVA, genus Vitivirus), with which it groups in all trees. The viral genome resembles that of trichoviruses but is smaller and does not contain the 3′ terminal fourth ORF found in some members of this genus. PTV may represent a new genus of plant viruses for which the provisional name of Andesvirus is proposed.

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Acknowledgments

The authors wish to thank Dr. B. D. Harrison, Scottish Crop Research Institute, Invergowrie, UK, for the many useful suggestions and the revision of the manuscript, and Dr. C. J. Jeffries, Potato Quarantine Unit, Scottish Agricultural Sciences Agency, Edinburgh, UK, for supplying the PVT isolate used in this study. The viral isolate was imported under permission of the UK Plant Health Service and the Italian Central Phytosanitary Service. Thanks are due also to Drs. E. V. Koonin, NCBI, Bethesda, MD, USA, for advice in the search of PVT conserved domains, and to Mrs. A. Antonacci for skillful technical assistance.

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Authors and Affiliations

  1. Istituto di Virologia Vegetale del CNR, sezione di Bari, Bari, Italy

    M. Russo, L. Rubino & A. De Stradis

  2. Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi, Via Amendola 165/A, 70126, Bari, Italy

    Giovanni P. Martelli

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  1. M. Russo
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  2. L. Rubino
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  3. A. De Stradis
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  4. Giovanni P. Martelli
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Correspondence to Giovanni P. Martelli.

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Russo, M., Rubino, L., De Stradis, A. et al. The complete nucleotide sequence of potato virus T. Arch Virol 154, 321–325 (2009). https://doi.org/10.1007/s00705-008-0300-x

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