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Complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus Ampelovirus

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Abstract

A new grapevine leafroll-associated virus isolate (GLRaV-Pr) from Greek grapevines was recently reported. This virus, along with the genetically related GLRaV-4, -5, -6 and -9, form a separate diverse lineage within the genus Ampelovirus. In this paper, the complete nucleotide sequence of GLRaV-Pr was determined, making it the first fully sequenced virus of this lineage. Its genome is 13,696 nt long and contains seven open reading frames, which potentially encode a 253-kDa polyprotein containing papain-like protease, methyltransferase, AlkB and helicase domains, a 58.2-kDa RNA-dependent RNA polymerase, a 5.2-kDa hydrophobic protein, a 58.5-kDa heat shock 70 protein homologue, a 60-kDa protein, a 30-kDa coat protein (CP) and a 23-kDa protein. A virus-specific antibody was raised against the recombinant CP of GLRaV-Pr and was applied in western blot analysis. The genomic, serological and phylogenetic data reported here confirm that GLRaV-Pr is a member of a distinct Ampelovirus species. Comparisons of GLRaV-Pr with the only available genetically related, fully sequenced virus, PMWaV-1, PBNSPaV and the partially sequenced GLRaV-9 revealed that this lineage, including GLRaV-4, -5, -6, -9 and -De, exhibits a high uniformity of genome organization and includes the smallest and simplest viruses within the family Closteroviridae.

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Acknowledgments

This work was financed by a research project for the reinforcement of new scientists [PENED 2003, funded by 75% of the public cost from the European Union, 25% from the Greek state (Ministry of Development-General Secretariat of Research and Technology, GSRT) and from the private sector (E.P.A.N.-Third European Community Framework)]. The authors wish to thank Dr. P. Gugerli (Agroscope Changins-Wadenswil Research Station ACW, Switzerland) for providing the GLRaV-9 isolate and also H. Bouzalas and E. Chatzinasiou (Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine) for their help with rabbit immunization.

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Authors and Affiliations

  1. Plant Pathology Laboratory, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Varvara I. Maliogka, L. Lotos, K. Efthimiou & N. I. Katis

  2. Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    C. I. Dovas

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  1. Varvara I. Maliogka
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Correspondence to N. I. Katis.

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Maliogka, V.I., Dovas, C.I., Lotos, L. et al. Complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus Ampelovirus . Arch Virol 154, 209–218 (2009). https://doi.org/10.1007/s00705-008-0290-8

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